PMID- 22779064
OWN - NLM
STAT- MEDLINE
DCOM- 20121030
LR  - 20240315
IS  - 2234-3814 (Electronic)
IS  - 2234-3806 (Print)
IS  - 2234-3806 (Linking)
VI  - 32
IP  - 4
DP  - 2012 Jul
TI  - Trueness assessment for serum glucose measurement using commercial systems 
      through the preparation of commutable reference materials.
PG  - 243-9
LID - 10.3343/alm.2012.32.4.243 [doi]
AB  - BACKGROUND: Commutable reference materials (RMs) are suitable for end-users for 
      evaluating the metrological traceability of values obtained using routine 
      measurement systems. We assessed the performance of 6 routine measurement systems 
      with validated secondary RMs. METHODS: We tested the homogeneity, stability, and 
      commutability of 5 minimally processed human serum pools according to the 
      standard guidelines. The serum pools were assigned values as per the reference 
      procedure of the United States Centers for Disease Control and were used to 
      evaluate the trueness of results from 6 commercial measurement systems based on 
      enzymatic methods: 3 glucose oxidase (GOD) and 3 hexokinase (HK) methods. 
      RESULTS: The prepared RMs were validated to be sufficiently homogenous, stable, 
      and commutable with the patient samples. Method bias varied for different 
      systems: GOD01, -0.17 to 2.88%; GOD02, 1.66 to 4.58%; GOD03, -0.17 to 3.14%; 
      HK01, -3.48 to -0.85%; HK02, -3.83 to -0.11%, and HK03, -1.82 to -0.27%. 
      CONCLUSIONS: We observed that the prepared serum glucose RMs were qualified for 
      trueness assessment. Most of the measurement systems met the minimal quality 
      specifications.
FAU - Xia, Changyu
AU  - Xia C
AD  - Department of Clinical Laboratory, Peking University First Hospital, Beijing, 
      China.
FAU - Liu, Ou
AU  - Liu O
FAU - Wang, Lanzhen
AU  - Wang L
FAU - Xu, Guobing
AU  - Xu G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120620
PL  - Korea (South)
TA  - Ann Lab Med
JT  - Annals of laboratory medicine
JID - 101571172
RN  - 0 (Blood Glucose)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - EC 1.1.3.4 (Glucose Oxidase)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Blood Chemical Analysis/instrumentation/*standards
MH  - Blood Glucose/*analysis
MH  - Glucose Oxidase/metabolism
MH  - Hexokinase/metabolism
MH  - Humans
MH  - Reagent Kits, Diagnostic
MH  - Reference Standards
MH  - Regression Analysis
PMC - PMC3384804
OTO - NOTNLM
OT  - Bias
OT  - Commercial system
OT  - Glucose
OT  - Reference material
COIS- No potential conflicts of interest relevant to this article were reported.
EDAT- 2012/07/11 06:00
MHDA- 2012/10/31 06:00
PMCR- 2012/07/01
CRDT- 2012/07/11 06:00
PHST- 2011/12/27 00:00 [received]
PHST- 2012/03/30 00:00 [revised]
PHST- 2012/05/18 00:00 [accepted]
PHST- 2012/07/11 06:00 [entrez]
PHST- 2012/07/11 06:00 [pubmed]
PHST- 2012/10/31 06:00 [medline]
PHST- 2012/07/01 00:00 [pmc-release]
AID - 10.3343/alm.2012.32.4.243 [doi]
PST - ppublish
SO  - Ann Lab Med. 2012 Jul;32(4):243-9. doi: 10.3343/alm.2012.32.4.243. Epub 2012 Jun 
      20.