PMID- 22781706
OWN - NLM
STAT- MEDLINE
DCOM- 20121022
LR  - 20181201
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 91
IP  - 5-6
DP  - 2012 Sep 4
TI  - Hepatoprotective and anti-inflammatory effects of silibinin on experimental 
      preeclampsia induced by L-NAME in rats.
PG  - 159-65
LID - 10.1016/j.lfs.2012.06.036 [doi]
AB  - AIMS: Inhibition of nitric oxide synthase with N-omega-nitro-l-arginine methyl 
      ester (l-NAME) has been employed as an experimental model of human preeclampsia. 
      This study determined the protective effect of silibinin, a flavonoid with 
      anti-inflammatory and hepatoprotective properties on the deleterious effects 
      observed in experimentally induced preeclampsia in rats. MAIN METHODS: Pregnant 
      Wistar rats were treated during gestation (days 10-20) with l-NAME 
      (70-80mg/kg/day) in drinking water or with l-NAME plus silibinin (100mg/kg/day, 
      orally) starting at day 0, day 7 or day 14 of pregnancy. Systolic blood pressure 
      was recorded from gestation days 0 to 21. A control group of pregnant non-treated 
      rats was analyzed similarly. On day 21 the rats were euthanized and the following 
      parameters were evaluated: proteinuria, platelet count, liver histopathology and 
      reproductive outcome. Tumor necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1beta), 
      IL-6, IL-10 and interferon-gamma (IFN-gamma) were determined in liver homogenate by 
      enzyme immunoassay. KEY FINDINGS: In comparison with the l-NAME group the 
      silibinin treatment reduced the values of systolic blood pressure, proteinuria, 
      TNF-alpha, IL-1beta and IFN-gamma in liver, normalized the platelet count and improved fetal 
      outcomes. Histopathological lesions in liver of the l-NAME group showed intense 
      mononuclear inflammatory infiltrate and thickening of muscle tunica of arterial 
      vessel, mainly in the periportal area. Silibinin treatment induced attenuation of 
      periportal inflammatory infiltrate, showing an association between inflammatory 
      infiltrate and TNF-alpha, IL-1beta and IFN-gamma levels in liver homogenate. SIGNIFICANCE: 
      Silibinin administration to l-NAME-treated rats displays anti-inflammatory and 
      immunomodulatory actions that may contribute to its hepatoprotective effects and 
      improve reproductive outcomes in experimental preeclampsia.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Souza, Camila Oliveira de
AU  - Souza CO
AD  - Department of Gynecology and Obstetrics, Botucatu Medical School, Sao Paulo State 
      University, Botucatu, Sao Paulo, SP, Brazil.
FAU - Peracoli, Maria Terezinha Serrao
AU  - Peracoli MT
FAU - Weel, Ingrid Cristina
AU  - Weel IC
FAU - Bannwart, Camila Ferreira
AU  - Bannwart CF
FAU - Romao, Mariana
AU  - Romao M
FAU - Nakaira-Takahagi, Erika
AU  - Nakaira-Takahagi E
FAU - Medeiros, Leonardo Teixeira Lopes de
AU  - Medeiros LT
FAU - Silva, Marcia Guimaraes da
AU  - Silva MG
FAU - Peracoli, Jose Carlos
AU  - Peracoli JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120707
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Immunologic Factors)
RN  - 0 (Protective Agents)
RN  - 0 (Silymarin)
RN  - 4RKY41TBTF (Silybin)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Blood Pressure/drug effects
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Immunologic Factors/pharmacology
MH  - Inflammation/*drug therapy/physiopathology
MH  - Liver/*drug effects/pathology
MH  - NG-Nitroarginine Methyl Ester
MH  - Pre-Eclampsia/*drug therapy
MH  - Pregnancy
MH  - Protective Agents/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Silybin
MH  - Silymarin/*pharmacology
EDAT- 2012/07/12 06:00
MHDA- 2012/10/23 06:00
CRDT- 2012/07/12 06:00
PHST- 2011/09/01 00:00 [received]
PHST- 2012/05/31 00:00 [revised]
PHST- 2012/06/25 00:00 [accepted]
PHST- 2012/07/12 06:00 [entrez]
PHST- 2012/07/12 06:00 [pubmed]
PHST- 2012/10/23 06:00 [medline]
AID - S0024-3205(12)00344-X [pii]
AID - 10.1016/j.lfs.2012.06.036 [doi]
PST - ppublish
SO  - Life Sci. 2012 Sep 4;91(5-6):159-65. doi: 10.1016/j.lfs.2012.06.036. Epub 2012 
      Jul 7.