PMID- 22781718
OWN - NLM
STAT- MEDLINE
DCOM- 20130829
LR  - 20131121
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 33
IP  - 4
DP  - 2012 Apr
TI  - [Cytogenetic analysis of childhood acute lymphoblastic leukemia].
PG  - 282-5
AB  - OBJECTIVE: To characterize the genetic aberrations in pediatric acute 
      lymphoblastic leukemia (ALL). METHODS: Ninety ALL cases were enrolled in the 
      study from January 2009 to November 2011. Chromosome banding analysis and 
      fluorescence in situ hybridization (FISH) were used to detect genetic 
      aberrations. RESULTS: (1) Chromosome analysis: 35 (53.0%) of 66 cases who had 
      metaphase were abnormal, and 24 cases had no metaphase. (2) FISH analysis: among 
      the 31 cases who had normal karyotypes and 24 who had no metaphase detected by 
      chromosome banding technique, 7 (22.6%) and 14 (58.3%) cases were abnormal 
      detected by FISH, respectively. There were no statistically significant 
      differences compared with chromosome analysis (P = 0.655). Among these 55 ALL 
      cases TEL/AML1, bcr-abl and MLL fusion genes were observed in 16 (29.1%), 3(5.5%) 
      and 2(3.6%) cases, respectively. (3) Cytogenetic aberration was observed in 56 of 
      total 90 ALL cases (62.2%). CONCLUSIONS: Cytogenetic changes are common in 
      childhood ALL. Conventional cytogenetic study could reliably detected chromosomal 
      abnormalities for ALL with assessable metaphase. FISH should be used as a 
      complementary method for ALL patients who have poor chromosomal morphology or no 
      metaphase cells, and combination of both methods can improve the detection rate 
      of genetic abnormalities in childhood leukemia.
FAU - Liu, Qing
AU  - Liu Q
AD  - Department of Hematology, Children's Hospital Affiliated to Shanghai Jiao Tong 
      University, Shanghai, China.
FAU - Jiang, Hui
AU  - Jiang H
FAU - Sun, Heng-juan
AU  - Sun HJ
FAU - Song, Yi-ju
AU  - Song YJ
FAU - Bao, Li-ming
AU  - Bao LM
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Aberrations
MH  - Female
MH  - Fusion Proteins, bcr-abl/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - *Karyotyping
MH  - Male
MH  - Myeloid-Lymphoid Leukemia Protein/genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
EDAT- 2012/07/12 06:00
MHDA- 2013/08/30 06:00
CRDT- 2012/07/12 06:00
PHST- 2012/07/12 06:00 [entrez]
PHST- 2012/07/12 06:00 [pubmed]
PHST- 2013/08/30 06:00 [medline]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2012 Apr;33(4):282-5.