PMID- 22782087
OWN - NLM
STAT- MEDLINE
DCOM- 20121105
LR  - 20151119
IS  - 1361-6528 (Electronic)
IS  - 0957-4484 (Linking)
VI  - 23
IP  - 30
DP  - 2012 Aug 3
TI  - Nanoparticles rapidly assess specific IgE in plasma.
PG  - 305101
LID - 10.1088/0957-4484/23/30/305101 [doi]
AB  - Allergy is the sixth leading cause of chronic disease in the world. This study 
      demonstrates the feasibility of detecting allergy indicators in human plasma, 
      noninvasively, at the point of care and with a comparable efficiency and reduced 
      turnaround time compared with the gold standard. Peanut allergy was utilized as a 
      model due to its widespread occurrence among the US population and fatality if 
      not treated. The detection procedure utilized magnetic nanoparticles that were 
      coated with an allergen layer (peanut protein extract). Peanut immunoglobulin E 
      (IgE) was detected in concentrations close to the minimum detection range of CAP 
      assay. The results were obtained in minutes compared with the CAP assay which 
      requires more than 3 h.
FAU - Ashraf, Sarmadia
AU  - Ashraf S
AD  - Center for Research Excellence in Nanobiosciences, University of North Carolina, 
      321 McIver Street Greensboro, NC 27412, USA.
FAU - Qadri, Shahnaz
AU  - Qadri S
FAU - al-Ramadi, Basel
AU  - al-Ramadi B
FAU - Haik, Yousef
AU  - Haik Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nanotechnology
JT  - Nanotechnology
JID - 101241272
RN  - 0 (Biomarkers)
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Plant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Absorption
MH  - Arachis/immunology
MH  - Biomarkers/blood
MH  - Enzyme-Linked Immunosorbent Assay/instrumentation/*methods
MH  - Humans
MH  - Immunoglobulin E/*blood/immunology
MH  - Magnetite Nanoparticles/*chemistry
MH  - Models, Immunological
MH  - Particle Size
MH  - Peanut Hypersensitivity/blood/immunology
MH  - Plant Proteins/immunology
MH  - Spectrometry, Fluorescence
EDAT- 2012/07/12 06:00
MHDA- 2012/11/06 06:00
CRDT- 2012/07/12 06:00
PHST- 2012/07/12 06:00 [entrez]
PHST- 2012/07/12 06:00 [pubmed]
PHST- 2012/11/06 06:00 [medline]
AID - 10.1088/0957-4484/23/30/305101 [doi]
PST - ppublish
SO  - Nanotechnology. 2012 Aug 3;23(30):305101. doi: 10.1088/0957-4484/23/30/305101.