PMID- 22782832
OWN - NLM
STAT- MEDLINE
DCOM- 20121113
LR  - 20120711
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 903
DP  - 2012
TI  - Protocol for analyzing human leukocyte antigen variants and sexually transmitted 
      infections: from genotyping to immunoassays.
PG  - 359-80
LID - 10.1007/978-1-61779-937-2_25 [doi]
AB  - This chapter describes experimental and analytical procedures that can be used to 
      decipher the specific role of human leukocyte antigen (HLA) variants in 
      infectious diseases. The techniques are distilled from more than one decade of 
      active immunogenetics research, primarily on sexually transmitted infections 
      (STIs) caused by viral and bacterial pathogens, including human immunodeficiency 
      virus type 1 (HIV-1), hepatitis B virus (HBV), and Chlamydia trachomatis. The 
      specific approaches cover (1) sequence-specific oligonucleotide (SSO) probe 
      hybridization for low-resolution genotyping, (2) sequencing-based typing (SBT) 
      for high-resolution, (3) statistical methods for testing associations between HLA 
      variants and phenotypic traits, and (4) enzyme-linked immunospot (ELISpot) assay 
      for enumerating HLA-restricted and epitope-specific T-lymphocyte responses. 
      Proper application of these mature and robust techniques should help establish 
      the importance of individual HLA alleles, haplotypes, and supertypes to 
      host-pathogen interactions.
FAU - Tang, Jianming
AU  - Tang J
AD  - Division of Infectious Diseases, University of Alabama at Birmingham, Birmingham, 
      AL, USA. jtang@uab.edu
FAU - Bansal, Anju
AU  - Bansal A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (HLA Antigens)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - *Genetic Variation
MH  - Genotyping Techniques/*methods
MH  - HLA Antigens/*genetics/*immunology
MH  - Humans
MH  - Immunoassay/*methods
MH  - Leukocytes, Mononuclear/immunology
MH  - Oligonucleotide Probes/genetics
MH  - Polymerase Chain Reaction
MH  - Sexually Transmitted Diseases/*genetics/*immunology
MH  - Th1 Cells/immunology
MH  - Th2 Cells/immunology
EDAT- 2012/07/12 06:00
MHDA- 2012/11/14 06:00
CRDT- 2012/07/12 06:00
PHST- 2012/07/12 06:00 [entrez]
PHST- 2012/07/12 06:00 [pubmed]
PHST- 2012/11/14 06:00 [medline]
AID - 10.1007/978-1-61779-937-2_25 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2012;903:359-80. doi: 10.1007/978-1-61779-937-2_25.