PMID- 22784206
OWN - NLM
STAT- MEDLINE
DCOM- 20121121
LR  - 20220309
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Linking)
VI  - 122
IP  - 6
DP  - 2012 Sep
TI  - Extracellular signal-regulated kinase 1/2 signaling promotes oligodendrocyte 
      myelination in vitro.
PG  - 1167-80
LID - 10.1111/j.1471-4159.2012.07871.x [doi]
AB  - Multiple extracellular factors have been implicated in orchestrating myelination 
      of the CNS; however, less is known about the intracellular signaling cascades 
      that regulate this process. We have previously shown that brain-derived 
      neurotrophic factor (BDNF) promotes oligodendrocyte myelination. Here, we 
      screened for the activation of candidate signaling pathways in in vitro 
      myelination assays and found that extracellular signal-regulated kinase (Erk) 
      signaling positively correlated with basal levels of oligodendrocyte myelination 
      as well as BDNF-induced myelination in vitro. By selectively manipulating Erk1/2 
      activation in oligodendrocytes in vitro, we found that constitutive activation of 
      Erk1/2 significantly increased myelination, mimicking the promyelinating effect 
      of BDNF, and also caused myelination to occur earlier. Conversely, selective 
      inhibition of Erk1/2 in oligodendrocytes significantly reduced the basal level of 
      myelination and blocked the promyelinating effect of BDNF. Analysis of 
      myelinating spinal cord and corpus callosum white matter tracts revealed that the 
      majority of mature oligodendrocytes are co-labeled with phospho-Erk1/2, whereas 
      phospho-Erk1/2 was rarely observed in oligodendrocyte progenitor cells. Finally, 
      the total level of phospho-Erk1/2 correlated with myelin formation during the 
      early postnatal period. Collectively, these data identify that Erk1/2 signaling 
      within oligodendrocytes exerts an important and direct effect to promote 
      myelination.
CI  - (c) 2012 The Authors. Journal of Neurochemistry (c) 2012 International Society for 
      Neurochemistry.
FAU - Xiao, Junhua
AU  - Xiao J
AD  - Centre for Neuroscience Research, The University of Melbourne, Victoria, 
      Australia. xiaoj@unimelb.edu.au
FAU - Ferner, Anita H
AU  - Ferner AH
FAU - Wong, Agnes W
AU  - Wong AW
FAU - Denham, Mark
AU  - Denham M
FAU - Kilpatrick, Trevor J
AU  - Kilpatrick TJ
FAU - Murray, Simon S
AU  - Murray SS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120803
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism/physiology
MH  - Cell Communication/physiology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Female
MH  - MAP Kinase Signaling System/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinase 3/physiology
MH  - Myelin Sheath/*physiology
MH  - Oligodendroglia/*cytology/*enzymology/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2012/07/13 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/07/13 06:00
PHST- 2012/07/13 06:00 [entrez]
PHST- 2012/07/13 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 10.1111/j.1471-4159.2012.07871.x [doi]
PST - ppublish
SO  - J Neurochem. 2012 Sep;122(6):1167-80. doi: 10.1111/j.1471-4159.2012.07871.x. Epub 
      2012 Aug 3.