PMID- 22784440
OWN - NLM
STAT- MEDLINE
DCOM- 20130704
LR  - 20181202
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 218
IP  - 2
DP  - 2013 Feb
TI  - Maturation of human iDCs by IL-18 plus PGE2, but not by each stimulus alone, 
      induced migration toward CCL21 and the secretion of IL-12 and IFN-gamma.
PG  - 238-44
LID - S0171-2985(12)00098-8 [pii]
LID - 10.1016/j.imbio.2012.05.003 [doi]
AB  - Dendritic cells (DCs) are potent antigen-presenting cells that initiate the 
      primary immune response and whose functional properties in vivo depend on the 
      maturation stimulus. We describe the functional properties of human 
      monocyte-derived DCs after the maturation of immature DCs (iDCs) for 2 days with 
      LPS (100 ng/ml), PGE2 (1 mug/ml), CD40L (1 mug/ml) or IL-18 (200 ng/ml) and with 
      CD40L+PGE2 and IL-18+PGE2 mixtures at the same concentrations as above. Neither 
      IL-18 nor PGE2 alone stimulated IL-12 or IFN-gamma secretion. When administered 
      simultaneously to 1x10(6)iDCs/ml, IL-18+PGE2 induced the secretion of 131.4+/-6.7 
      pg IL-12/ml and 355+/-87 pg IFN-gamma/ml but there was no detectable IL-10 secretion. 
      However, PGE2 alone stimulated the secretion of 208+/-89 pg IL-10/ml whereas IL-18 
      alone did not stimulate the secretion of IL-10, IL-12, TNF-alpha or INF-gamma. When the 
      mixture of CD40L+PGE2 was used, only migration toward CCL19 and CCL21 was 
      induced. CD40L did not stimulate the secretion of IL-10, IL-12, TNF-alpha or IFN-gamma 
      and did not stimulate migration toward CCL19 or CCL21. The extent of stimulation 
      of T cell proliferation was essentially the same for all stimuli at the 
      concentrations given above. New properties such as IL-12 and INF-gamma secretion and 
      migration toward CCL21 emerged when a mixture of IL-18+PGE2 was employed. These 
      data show that when the pairs of stimuli reported here were used simultaneously 
      their effect was not additive. This system can be used to prepare mDCs with 
      properties useful for cell therapy and also as a model to investigate the 
      mechanisms of cytokine secretion and cell migration.
CI  - Copyright (c) 2012 Elsevier GmbH. All rights reserved.
FAU - da Silva, Idalete
AU  - da Silva I
AD  - Departamento de Biologia Celular e Molecular e Bioagentes Patogenicos e Centro de 
      Quimica de Proteinas, Brazil.
FAU - Gomes, Glauce G
AU  - Gomes GG
FAU - Menezes, Camila C B O
AU  - Menezes CC
FAU - Palma, Patricia V B
AU  - Palma PV
FAU - Orellana, Maristela D
AU  - Orellana MD
FAU - Covas, Dimas T
AU  - Covas DT
FAU - Chammas, Roger
AU  - Chammas R
FAU - Greene, Lewis J
AU  - Greene LJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120511
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Chemokine CCL19)
RN  - 0 (Chemokine CCL21)
RN  - 0 (Interleukin-18)
RN  - 0 (Lipopolysaccharides)
RN  - 147205-72-9 (CD40 Ligand)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - CD40 Ligand/immunology
MH  - Cell Differentiation
MH  - Cell Movement/immunology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chemokine CCL19/metabolism
MH  - Chemokine CCL21/*metabolism
MH  - Dendritic Cells/*immunology/pathology
MH  - Dinoprostone/*immunology
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Interleukin-12/metabolism
MH  - Interleukin-18/*immunology
MH  - Lipopolysaccharides/immunology
MH  - Lymphocyte Activation
MH  - Monocytes/pathology
MH  - Receptor Cross-Talk
MH  - T-Lymphocytes/*immunology
EDAT- 2012/07/13 06:00
MHDA- 2013/07/05 06:00
CRDT- 2012/07/13 06:00
PHST- 2011/04/07 00:00 [received]
PHST- 2012/05/07 00:00 [accepted]
PHST- 2012/07/13 06:00 [entrez]
PHST- 2012/07/13 06:00 [pubmed]
PHST- 2013/07/05 06:00 [medline]
AID - S0171-2985(12)00098-8 [pii]
AID - 10.1016/j.imbio.2012.05.003 [doi]
PST - ppublish
SO  - Immunobiology. 2013 Feb;218(2):238-44. doi: 10.1016/j.imbio.2012.05.003. Epub 
      2012 May 11.