PMID- 22784633
OWN - NLM
STAT- MEDLINE
DCOM- 20121113
LR  - 20220224
IS  - 1432-0436 (Electronic)
IS  - 0301-4681 (Print)
IS  - 0301-4681 (Linking)
VI  - 84
IP  - 2
DP  - 2012 Sep
TI  - A uniform human Wnt expression library reveals a shared secretory pathway and 
      unique signaling activities.
PG  - 203-13
LID - 10.1016/j.diff.2012.06.004 [doi]
AB  - Wnt ligands are secreted morphogens that control multiple developmental processes 
      during embryogenesis and adult homeostasis. A diverse set of receptors and 
      signals have been linked to individual Wnts, but the lack of tools for 
      comparative analysis has limited the ability to determine which of these signals 
      are general for the entire Wnt family, and which define subsets of differently 
      acting ligands. We have created a versatile Gateway library of clones for all 19 
      human Wnts. An analysis comparing epitope-tagged and untagged versions of each 
      ligand shows that despite their similar expression at the mRNA level, Wnts 
      exhibit considerable variation in stability, processing and secretion. At least 
      14 out of the 19 Wnts activate beta-catenin-dependent signaling, an activity that is 
      cell type-dependent and tracks with the stabilization of beta-catenin and LRP6 
      phosphorylation. We find that the core Wnt modification and secretion proteins 
      Porcupine (PORCN) and Wntless (WLS) are essential for all Wnts to signal through 
      beta-catenin-dependent and independent pathways. This comprehensive toolkit provides 
      critical tools and new insights into human Wnt gene expression and function.
CI  - Copyright (c) 2012 International Society of Differentiation. Published by Elsevier 
      B.V. All rights reserved.
FAU - Najdi, Rani
AU  - Najdi R
AD  - Department of Microbiology and Molecular Genetics, University of California, 
      Irvine, CA, USA.
FAU - Proffitt, Kyle
AU  - Proffitt K
FAU - Sprowl, Stephanie
AU  - Sprowl S
FAU - Kaur, Simran
AU  - Kaur S
FAU - Yu, Jia
AU  - Yu J
FAU - Covey, Tracy M
AU  - Covey TM
FAU - Virshup, David M
AU  - Virshup DM
FAU - Waterman, Marian L
AU  - Waterman ML
LA  - eng
GR  - R01 CA108697/CA/NCI NIH HHS/United States
GR  - CA108697/CA/NCI NIH HHS/United States
GR  - P30 CA062203/CA/NCI NIH HHS/United States
GR  - R01 CA096878/CA/NCI NIH HHS/United States
GR  - CA096878/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120709
PL  - England
TA  - Differentiation
JT  - Differentiation; research in biological diversity
JID - 0401650
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (LRP6 protein, human)
RN  - 0 (Low Density Lipoprotein Receptor-Related Protein-6)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (WLS protein, human)
RN  - 0 (Wnt Proteins)
RN  - 0 (beta Catenin)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.- (PORCN protein, human)
SB  - IM
MH  - 3T3 Cells
MH  - Acyltransferases
MH  - Animals
MH  - Gene Expression Profiling
MH  - *Gene Library
MH  - HEK293 Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/metabolism
MH  - Low Density Lipoprotein Receptor-Related Protein-6/metabolism
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Receptors, G-Protein-Coupled/metabolism
MH  - *Secretory Pathway
MH  - Wnt Proteins/genetics/*metabolism
MH  - *Wnt Signaling Pathway
MH  - beta Catenin/*metabolism
PMC - PMC4015730
MID - NIHMS571720
EDAT- 2012/07/13 06:00
MHDA- 2012/11/14 06:00
PMCR- 2014/05/09
CRDT- 2012/07/13 06:00
PHST- 2012/04/24 00:00 [received]
PHST- 2012/06/16 00:00 [accepted]
PHST- 2012/07/13 06:00 [entrez]
PHST- 2012/07/13 06:00 [pubmed]
PHST- 2012/11/14 06:00 [medline]
PHST- 2014/05/09 00:00 [pmc-release]
AID - S0301-4681(12)00087-4 [pii]
AID - 10.1016/j.diff.2012.06.004 [doi]
PST - ppublish
SO  - Differentiation. 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 
      2012 Jul 9.