PMID- 22786889
OWN - NLM
STAT- MEDLINE
DCOM- 20130110
LR  - 20220310
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 160
IP  - 1
DP  - 2012 Sep
TI  - The lateral organ boundaries domain transcription factor LBD20 functions in 
      Fusarium wilt Susceptibility and jasmonate signaling in Arabidopsis.
PG  - 407-18
LID - 10.1104/pp.112.199067 [doi]
AB  - The LATERAL ORGAN BOUNDARIES (LOB) DOMAIN (LBD) gene family encodes 
      plant-specific transcriptional regulators functioning in organ development. In a 
      screen of Arabidopsis (Arabidopsis thaliana) sequence-indexed transferred DNA 
      insertion mutants, we found disruption of the LOB DOMAIN-CONTAINING PROTEIN20 
      (LBD20) gene led to increased resistance to the root-infecting vascular wilt 
      pathogen Fusarium oxysporum. In wild-type plants, LBD20 transcripts were barely 
      detectable in leaves but abundant in roots, where they were further induced after 
      F. oxysporum inoculation or methyl jasmonate treatment. Induction of LBD20 
      expression in roots was abolished in coronatine insensitive1 (coi1) and myc2 
      (allelic to jasmonate insensitive1) mutants, suggesting LBD20 may function in 
      jasmonate (JA) signaling. Consistent with this, expression of the JA-regulated 
      THIONIN2.1 (Thi2.1) and VEGETATIVE STORAGE PROTEIN2 (VSP2) genes were 
      up-regulated in shoots of lbd20 following treatment of roots with F. oxysporum or 
      methyl jasmonate. However, PLANT DEFENSIN1.2 expression was unaltered, indicating 
      a repressor role for LBD20 in a branch of the JA-signaling pathway. Plants 
      overexpressing LBD20 (LBD20-OX) had reduced Thi2.1 and VSP2 expression. There was 
      a significant correlation between increased LBD20 expression in the LBD20-OX 
      lines with both Thi2.1 and VSP2 repression, and reduced survival following F. 
      oxysporum infection. Chlorosis resulting from application of F. oxysporum culture 
      filtrate was also reduced in lbd20 leaves relative to the wild type. Taken 
      together, LBD20 is a F. oxysporum susceptibility gene that appears to regulate 
      components of JA signaling downstream of COI1 and MYC2 that are required for full 
      elicitation of F. oxysporum- and JA-dependent responses. To our knowledge, this 
      is the first demonstration of a role for a LBD gene family member in either 
      biotic stress or JA signaling.
FAU - Thatcher, Louise F
AU  - Thatcher LF
AD  - Commonwealth Scientific and Industrial Research Organization Plant Industry, 
      Queensland Bioscience Precinct, St. Lucia, Brisbane, Queensland 4067, Australia. 
      louise.thatcher@csiro.au
FAU - Powell, Jonathan J
AU  - Powell JJ
FAU - Aitken, Elizabeth A B
AU  - Aitken EA
FAU - Kazan, Kemal
AU  - Kazan K
FAU - Manners, John M
AU  - Manners JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120710
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Acetates)
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors)
RN  - 0 (COI1 protein, Arabidopsis)
RN  - 0 (Culture Media)
RN  - 0 (Cyclopentanes)
RN  - 0 (MYC2 protein, Arabidopsis)
RN  - 0 (Oxylipins)
RN  - 0 (Repressor Proteins)
RN  - 0 (VSP2 protein, Arabidopsis)
RN  - 900N171A0F (methyl jasmonate)
SB  - IM
MH  - Acetates/*pharmacology
MH  - Alleles
MH  - Arabidopsis/genetics/immunology/*microbiology
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/genetics/metabolism
MH  - Culture Media/metabolism
MH  - Cyclopentanes/*pharmacology
MH  - Disease Susceptibility/immunology
MH  - Fusarium/immunology/*pathogenicity
MH  - Gene Expression Regulation, Plant
MH  - Oxylipins/*pharmacology
MH  - Plant Diseases/immunology/microbiology
MH  - Plant Roots/immunology/microbiology
MH  - Repressor Proteins/genetics/metabolism
MH  - *Signal Transduction
MH  - Stress, Physiological
PMC - PMC3440215
EDAT- 2012/07/13 06:00
MHDA- 2013/01/11 06:00
PMCR- 2013/09/01
CRDT- 2012/07/13 06:00
PHST- 2012/07/13 06:00 [entrez]
PHST- 2012/07/13 06:00 [pubmed]
PHST- 2013/01/11 06:00 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - pp.112.199067 [pii]
AID - 199067 [pii]
AID - 10.1104/pp.112.199067 [doi]
PST - ppublish
SO  - Plant Physiol. 2012 Sep;160(1):407-18. doi: 10.1104/pp.112.199067. Epub 2012 Jul 
      10.