PMID- 22790512
OWN - NLM
STAT- MEDLINE
DCOM- 20121010
LR  - 20211021
IS  - 1432-1211 (Electronic)
IS  - 0093-7711 (Linking)
VI  - 64
IP  - 8
DP  - 2012 Aug
TI  - Study of cynomolgus monkey (Macaca fascicularis) Mhc DRB gene polymorphism in 
      four populations.
PG  - 605-14
LID - 10.1007/s00251-012-0613-5 [doi]
AB  - The cynomolgus macaque (Macaca fascicularis) is currently used as an animal model 
      in various fields of immunology especially in the development of innovative 
      vaccines for the prevention and treatment of infectious diseases. The 
      polymorphism of the major histocompatibility complex (MHC) influences the 
      development of adaptive immune responses, and it is crucial to characterize the 
      polymorphism of cynomolgus MHC genes. Among all macaque species, the cynomolgus 
      macaque has the most diversified geographical area encompassing continental and 
      insular populations. By the study of a large sample of animals from the 
      Philippines (N = 359), we have characterized 20 DRB haplotypes. The DRB 
      genotyping was performed by denaturing gradient gel electrophoresis (DGGE) 
      sequencing of exon 2 and was confirmed by polymerase chain 
      reaction-sequence-specific oligonucleotide. The DRB and DRA cDNA of 126 animals 
      were characterized by cloning and sequencing. By means of DGGE sequencing, we 
      characterized the polymorphism of genomic DRB exon 2 in three other cynomolgus 
      macaque population samples (Java, Vietnam, and Mauritius), and we discuss about 
      the origin of the founders of the Mauritian and the Filipino cynomolgus macaque 
      populations.
FAU - Blancher, Antoine
AU  - Blancher A
AD  - Laboratoire d'Immunogenetique moleculaire, EA 3034, Faculte de Medecine Purpan, 
      Universite Paul Sabatier, Toulouse 3, CHU de Toulouse, Toulouse, France. 
      blancher.a@chu-toulouse.fr
FAU - Aarnink, Alice
AU  - Aarnink A
FAU - Tanaka, Keiko
AU  - Tanaka K
FAU - Ota, Masao
AU  - Ota M
FAU - Inoko, Hidetoshi
AU  - Inoko H
FAU - Yamanaka, Hisashi
AU  - Yamanaka H
FAU - Nakagawa, Hiroshi
AU  - Nakagawa H
FAU - Apoil, Pol-Andre
AU  - Apoil PA
FAU - Shiina, Takashi
AU  - Shiina T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120404
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
SB  - IM
MH  - Animals
MH  - Exons
MH  - *Genes, MHC Class II
MH  - Genotype
MH  - Haplotypes
MH  - Macaca fascicularis/*genetics
MH  - Philippines
MH  - Phylogeny
MH  - Polymorphism, Genetic
MH  - Sequence Analysis, DNA
EDAT- 2012/07/14 06:00
MHDA- 2012/10/12 06:00
CRDT- 2012/07/14 06:00
PHST- 2012/01/18 00:00 [received]
PHST- 2012/03/12 00:00 [accepted]
PHST- 2012/07/14 06:00 [entrez]
PHST- 2012/07/14 06:00 [pubmed]
PHST- 2012/10/12 06:00 [medline]
AID - 10.1007/s00251-012-0613-5 [doi]
PST - ppublish
SO  - Immunogenetics. 2012 Aug;64(8):605-14. doi: 10.1007/s00251-012-0613-5. Epub 2012 
      Apr 4.