PMID- 22790944
OWN - NLM
STAT- MEDLINE
DCOM- 20121105
LR  - 20161125
IS  - 1347-6947 (Electronic)
IS  - 0916-8451 (Linking)
VI  - 76
IP  - 6
DP  - 2012
TI  - Identification of a new IgE-binding epitope of peanut oleosin that cross-reacts 
      with buckwheat.
PG  - 1182-8
AB  - Peanut and buckwheat induce a severe allergic reaction, anaphylaxis, which is 
      considered to be mediated by immunoglobulin E (IgE). We identified in this study 
      a new IgE-binding epitope of the peanut allergen that cross-reacted with 
      buckwheat. The phosphate-buffered saline-soluble fraction of buckwheat inhibited 
      the binding between IgE and the peanut allergen. A cross-reactive peptide was 
      isolated from the alpha-chymotrypsin hydrolysate of peanut. Based on the amino acid 
      sequence and mass spectrometric analysis data, the peptide was identified as 
      Ser-Asp-Gln-Thr-Arg-Thr-Gly-Tyr (SDQTRTGY); this sequence is identical to amino 
      acids 2-9 in the N-terminal hydrophilic domain of oleosin 3 which is located on 
      the surface of the lipid storage body. Synthetic SDQTRTGY was found to bind with 
      IgE in the sera of all eight peanut-allergic patients tested. Since many foods of 
      plant origin contain oleosin, the possibility of an anaphylactic cross-reaction 
      in allergic patients should always be considered.
FAU - Kobayashi, Shoko
AU  - Kobayashi S
AD  - Research Center for Food Safety, Department of Applied Biological Chemistry, 
      Graduate School of Agricultural and Life Sciences, The University of Tokyo, 
      Tokyo, Japan. ashoko@mail.ecc.u-tokyo.ac.jp
FAU - Katsuyama, Shinta
AU  - Katsuyama S
FAU - Wagatsuma, Tamae
AU  - Wagatsuma T
FAU - Okada, Shinji
AU  - Okada S
FAU - Tanabe, Soichi
AU  - Tanabe S
LA  - eng
PT  - Journal Article
DEP - 20120607
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Buffers)
RN  - 0 (Plant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.21.1 (Chymotrypsin)
RN  - EC 3.4.21.1 (alpha-chymotrypsin)
SB  - IM
MH  - Allergens/*chemistry/immunology
MH  - Amino Acid Sequence
MH  - Antigens, Plant/*chemistry/immunology
MH  - Arachis/*chemistry/immunology
MH  - Binding, Competitive
MH  - Buffers
MH  - Chymotrypsin/chemistry
MH  - Cross Reactions
MH  - Fagopyrum/*chemistry/immunology
MH  - Humans
MH  - Immunoglobulin E/*chemistry/immunology
MH  - Molecular Sequence Data
MH  - Peanut Hypersensitivity/*immunology
MH  - Plant Proteins/*chemistry/immunology
MH  - Protein Structure, Tertiary
MH  - Proteolysis
MH  - Sequence Homology, Amino Acid
EDAT- 2012/07/14 06:00
MHDA- 2012/11/06 06:00
CRDT- 2012/07/14 06:00
PHST- 2012/07/14 06:00 [entrez]
PHST- 2012/07/14 06:00 [pubmed]
PHST- 2012/11/06 06:00 [medline]
AID - DN/JST.JSTAGE/bbb/120063 [pii]
AID - 10.1271/bbb.120063 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2012;76(6):1182-8. doi: 10.1271/bbb.120063. Epub 2012 
      Jun 7.