PMID- 22791305
OWN - NLM
STAT- MEDLINE
DCOM- 20130415
LR  - 20181201
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 90
IP  - 11
DP  - 2012 Nov
TI  - Intracerebral transplantation of bone marrow stromal cells ameliorates tissue 
      plasminogen activator-induced brain damage after cerebral ischemia in mice 
      detected by in vivo and ex vivo optical imaging.
PG  - 2086-93
LID - 10.1002/jnr.23104 [doi]
AB  - Detection and protection of the neurovascular unit (NVU) are essential for 
      treatment of acute stroke patients, especially the use of tissue plasminogen 
      activator (tPA). In the present study, we conducted in vivo and ex vivo optical 
      imaging for detecting activation of matrix metalloproteinases (MMPs) and 
      evaluated the protective effect of intracerebral transplantation of bone marrow 
      stromal cells (BMSCs) obtained from green fluorescent protein (GFP) transgenic 
      (Tg) mice on the NVU in tPA-mediated brain damage after transient middle cerebral 
      artery occlusion (tMCAO) in mice. Compared with the tMCAO group, the tMCAO plus 
      BMSC group showed significant reductions of in vivo and ex vivo fluorescent 
      signals for MMPs at 48 hr after tMCAO, with a partial colocalization of BMSC-GFP 
      signals. Intracerebrally transplanted BMSCs ameliorated MMP-9 activation by 
      immunohistochemistry and Western blot with differentiation into microglial and 
      astroglial cells. Double-immunofluorescence study revealed improved NVU 
      disruption in the tMCAO plus BMSC group. The present study suggests that 
      intracerebral BMSC transplantation reduced MMP activation and subsequent NVU 
      disruption caused by tPA after tMCAO and that this MMP activation and BMSC effect 
      were detectable with in vivo and ex vivo optical imaging.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Liu, Ning
AU  - Liu N
AD  - Department of Neurology, Okayama University Graduate School of Medicine, 
      Dentistry and Pharmaceutical Sciences, Okayama, Japan.
FAU - Deguchi, Kentaro
AU  - Deguchi K
FAU - Yamashita, Toru
AU  - Yamashita T
FAU - Liu, Wentao
AU  - Liu W
FAU - Ikeda, Yoshio
AU  - Ikeda Y
FAU - Abe, Koji
AU  - Abe K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120713
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Fibrinolytic Agents)
RN  - EC 3.4.21.68 (Tissue Plasminogen Activator)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - *Bone Marrow Transplantation
MH  - Brain Ischemia/metabolism/*therapy
MH  - Cerebral Hemorrhage/chemically induced/therapy
MH  - Enzyme Activation/drug effects
MH  - Fibrinolytic Agents/*toxicity
MH  - Fluorescent Antibody Technique
MH  - Male
MH  - Matrix Metalloproteinases/biosynthesis
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Optical Imaging
MH  - Tissue Plasminogen Activator/*toxicity
EDAT- 2012/07/14 06:00
MHDA- 2013/04/16 06:00
CRDT- 2012/07/14 06:00
PHST- 2012/03/13 00:00 [received]
PHST- 2012/05/04 00:00 [revised]
PHST- 2012/05/24 00:00 [accepted]
PHST- 2012/07/14 06:00 [entrez]
PHST- 2012/07/14 06:00 [pubmed]
PHST- 2013/04/16 06:00 [medline]
AID - 10.1002/jnr.23104 [doi]
PST - ppublish
SO  - J Neurosci Res. 2012 Nov;90(11):2086-93. doi: 10.1002/jnr.23104. Epub 2012 Jul 
      13.