PMID- 22794168
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121106
LR  - 20211021
IS  - 1755-8166 (Electronic)
IS  - 1755-8166 (Linking)
VI  - 5
IP  - 1
DP  - 2012 Jul 16
TI  - Array-based comparative genomic hybridization is more informative than 
      conventional karyotyping and fluorescence in situ hybridization in the analysis 
      of first-trimester spontaneous abortion.
PG  - 33
LID - 10.1186/1755-8166-5-33 [doi]
AB  - BACKGROUND: Array-based comparative genomic hybridization (aCGH) is a new 
      technique for detecting submicroscopic deletions and duplications, and can 
      overcome many of the limitations associated with classic cytogenetic analysis. 
      However, its clinical use in spontaneous abortion needs comprehensive evaluation. 
      We used aCGH to investigate chromosomal imbalances in 100 spontaneous abortions 
      and compared the results with G-banding karyotyping and fluorescence in situ 
      hybridization (FISH). Inconsistent results were verified by quantitative 
      fluorescence PCR. RESULTS: Abnormalities were detected in 61 cases. aCGH achieved 
      the highest detection rate (93.4%, 57/61) compared with traditional karyotyping 
      (77%, 47/61) and FISH analysis (68.9%, 42/61). aCGH identified all chromosome 
      abnormalities reported by traditional karyotyping and interphase FISH analysis, 
      with the exception of four triploids. It also detected three additional 
      aneuploidy cases in 37 specimens with 'normal' karyotypes, one mosaicism and 10 
      abnormalities in 14 specimens that failed to grow in vitro. CONCLUSIONS: aCGH 
      analysis circumvents many limitations in traditional karyotyping or FISH. The 
      accuracy and efficiency of aCGH in spontaneous abortions highlights its clinical 
      usefulness for the future. As aborted tissues have the potential to be 
      contaminated with maternal cells, the threshold value of detection in aCGH should 
      be lowered to avoid false negatives.
FAU - Gao, Jinsong
AU  - Gao J
AD  - Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, 
      Chinese Academy of Medical Science, Shuai Fu Yuan No,1, Dongdan, Beijing 100730, 
      People's Republic of China. Liu_jt@sohu.com.
FAU - Liu, Congcong
AU  - Liu C
FAU - Yao, Fengxia
AU  - Yao F
FAU - Hao, Na
AU  - Hao N
FAU - Zhou, Jing
AU  - Zhou J
FAU - Zhou, Qian
AU  - Zhou Q
FAU - Zhang, Liang
AU  - Zhang L
FAU - Liu, Xinyan
AU  - Liu X
FAU - Bian, Xuming
AU  - Bian X
FAU - Liu, Juntao
AU  - Liu J
LA  - eng
PT  - Journal Article
DEP - 20120716
PL  - England
TA  - Mol Cytogenet
JT  - Molecular cytogenetics
JID - 101317942
PMC - PMC3488553
EDAT- 2012/07/17 06:00
MHDA- 2012/07/17 06:01
PMCR- 2012/07/16
CRDT- 2012/07/17 06:00
PHST- 2012/04/12 00:00 [received]
PHST- 2012/05/20 00:00 [accepted]
PHST- 2012/07/17 06:00 [entrez]
PHST- 2012/07/17 06:00 [pubmed]
PHST- 2012/07/17 06:01 [medline]
PHST- 2012/07/16 00:00 [pmc-release]
AID - 1755-8166-5-33 [pii]
AID - 10.1186/1755-8166-5-33 [doi]
PST - epublish
SO  - Mol Cytogenet. 2012 Jul 16;5(1):33. doi: 10.1186/1755-8166-5-33.