PMID- 22796097
OWN - NLM
STAT- MEDLINE
DCOM- 20130124
LR  - 20220311
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 26
IP  - 7
DP  - 2012 Oct
TI  - Prediction of skin sensitization potency of chemicals by human Cell Line 
      Activation Test (h-CLAT) and an attempt at classifying skin sensitization 
      potency.
PG  - 1150-60
LID - 10.1016/j.tiv.2012.07.001 [doi]
AB  - The human Cell Line Activation Test (h-CLAT), an in vitro skin sensitization 
      test, is based on the augmentation of CD86 and CD54 expression in THP-1 cells 
      following exposure to chemicals. The h-CLAT was found to be capable of 
      determining the hazard of skin sensitization. In contrast, the local lymph node 
      assay (LLNA), widely used as a stand-alone method in Europe and US, identifies 
      the same hazard, but also classifies the potency by using the estimated 
      concentration of SI=3 (EC3). In this study, several values calculated from the 
      h-CLAT data were evaluated for its correlation to the LLNA EC3 determination. A 
      statistically significant correlation was observed between h-CLAT concentration 
      providing a cell viability of 75% (CV75), h-CLAT estimated concentration of 
      RFI=150 for CD86 (EC150), and for CD54 (EC200) with LLNA's EC3. From EC150 and 
      EC200, a minimum induction threshold (MIT) was determined as the smaller of 
      either EC150 or EC200. MIT showed a correlation with EC3 (R=0.638). Also, MIT had 
      an approximate 80% accuracy for sub-categories of the globally harmonized system 
      (GHS) when a tentative threshold of 13 mug/mL was used. From these data, the 
      h-CLAT values may be one of the useful tools to predict the allergic potency of 
      chemicals.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Nukada, Yuko
AU  - Nukada Y
AD  - Safety Science Research Laboratories, Kao Corporation, 2606 Akabane, 
      Ichikai-machi, Haga-gun, Tochigi 321-3497, Japan. nukada.yuko@kao.co.jp
FAU - Ashikaga, Takao
AU  - Ashikaga T
FAU - Miyazawa, Masaaki
AU  - Miyazawa M
FAU - Hirota, Morihiko
AU  - Hirota M
FAU - Sakaguchi, Hitoshi
AU  - Sakaguchi H
FAU - Sasa, Hitoshi
AU  - Sasa H
FAU - Nishiyama, Naohiro
AU  - Nishiyama N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20120710
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Allergens)
SB  - IM
MH  - Allergens/classification/*toxicity
MH  - Animal Testing Alternatives/*methods
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Dermatitis, Contact/*etiology/immunology/pathology
MH  - Humans
MH  - Hypersensitivity/*etiology/immunology/pathology
MH  - Local Lymph Node Assay
MH  - Monocytes/*drug effects/immunology/pathology
MH  - Predictive Value of Tests
MH  - Reproducibility of Results
MH  - Risk Assessment
MH  - *Skin Irritancy Tests
EDAT- 2012/07/17 06:00
MHDA- 2013/01/25 06:00
CRDT- 2012/07/17 06:00
PHST- 2012/02/28 00:00 [received]
PHST- 2012/06/08 00:00 [revised]
PHST- 2012/07/03 00:00 [accepted]
PHST- 2012/07/17 06:00 [entrez]
PHST- 2012/07/17 06:00 [pubmed]
PHST- 2013/01/25 06:00 [medline]
AID - S0887-2333(12)00192-0 [pii]
AID - 10.1016/j.tiv.2012.07.001 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2012 Oct;26(7):1150-60. doi: 10.1016/j.tiv.2012.07.001. Epub 
      2012 Jul 10.