PMID- 22797215
OWN - NLM
STAT- MEDLINE
DCOM- 20121126
LR  - 20171116
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 138
IP  - 1
DP  - 2012
TI  - Heterochromatin, sex chromosomes and rRNA gene clusters in Coprophanaeus beetles 
      (Coleoptera, Scarabaeidae).
PG  - 46-55
LID - 10.1159/000339648 [doi]
AB  - Repetitive DNA sequences constitute a high fraction of eukaryotic genomes and are 
      considered a key component for the chromosome and karyotype evolution. For a 
      better understanding of their evolutionary role in beetles, we examined the 
      chromosomes of 5 species of the genus Coprophanaeus by C-banding, fluorochrome 
      staining CMA(3)/DA/DAPI, and fluorescence in situ hybridization (FISH) with probes 
      for 18S and 5S rRNA genes. The Coprophanaeus species have identical chromosome 
      numbers and a conserved chromosome morphology. However, they show different sex 
      chromosome forms, XY, Xy, XY(p), and heterochromatin seems to be involved in the 
      origin and diversification of these forms. C-banding showed primarily the 
      presence of diphasic chromosomes in all species examined. After CMA(3)/DA/DAPI 
      staining, 1-9 autosomal pairs showed CMA(3)-positive blocks depending on the 
      species, while DAPI-positive blocks were detected only in Coprophanaeusdardanus. 
      FISH mapping revealed 5S rDNA signals in one autosomal pair in each species, 
      whereas the number of pairs with 18S rDNA signals varied from 1-8 between the 
      Coprophanaeus species. Our results suggest that distinct genetic mechanisms had 
      been involved in the karyotype evolution of Coprophanaeus species, i.e. 
      mechanisms maintaining the conserved number of 5S rDNA clusters and those 
      generating variability in the amount of heterochromatin, sex chromosome forms, 
      and distribution of 18S rDNA clusters.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - Oliveira, S G
AU  - Oliveira SG
AD  - Departamento de Morfologia, Instituto de Biociencias, Universidade Estadual 
      Paulista/UNESP, Botucatu, Brazil.
FAU - Cabral-de-Mello, D C
AU  - Cabral-de-Mello DC
FAU - Arcanjo, A P
AU  - Arcanjo AP
FAU - Xavier, C
AU  - Xavier C
FAU - Souza, M J
AU  - Souza MJ
FAU - Martins, C
AU  - Martins C
FAU - Moura, R C
AU  - Moura RC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120711
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Animals
MH  - Coleoptera/*genetics
MH  - DNA, Ribosomal/genetics
MH  - *Genes, rRNA
MH  - *Heterochromatin
MH  - Male
MH  - *Multigene Family
MH  - *Sex Chromosomes
EDAT- 2012/07/17 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/07/17 06:00
PHST- 2012/05/09 00:00 [accepted]
PHST- 2012/07/17 06:00 [entrez]
PHST- 2012/07/17 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 000339648 [pii]
AID - 10.1159/000339648 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2012;138(1):46-55. doi: 10.1159/000339648. Epub 2012 Jul 
      11.