PMID- 22797767
OWN - NLM
STAT- MEDLINE
DCOM- 20121015
LR  - 20120814
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 137
IP  - 1
DP  - 2012
TI  - Integration of linkage and chromosome maps of red clover (Trifolium pratense L.).
PG  - 60-9
LID - 10.1159/000339509 [doi]
AB  - Red clover (Trifolium pratense L.) is a forage legume and an allogamous diploid 
      plant (2n = 14; 440 Mb). Here, we examine the 7 prometaphase chromosomes of red 
      clover using fluorescence in situ hybridization (FISH) with ribosomal RNA 
      sequences, pericentromeric and telomeric repeats, as well as bacterial artificial 
      chromosome (BAC) clones. Position of hybridization signals and chromosome 
      condensation patterns were quantified by the help of the chromosome image 
      analysis system ver. 4.0 (CHIAS IV). Fourteen BAC clones belonging to linkage 
      groups (LG) 1-7 hybridized to individual chromosomes 4, 2, 6, 5, 1, 7, and 3, 
      respectively. Quantitative analysis of FISH mapping and chromosome analysis using 
      CHIAS IV allowed us to construct a quantitative idiogram that constitutes the 
      comprehensive chromosome map of red clover. Chromosomal positions of the 26S rDNA 
      locus were detected at a heterozygous locus on chromosome 6 in the variety HR, 
      and polymorphisms of rDNA loci were observed in other varieties, although 
      chromosomal positions of some BAC clones did not vary among HR and other 
      varieties. These results demonstrate chromosomal collinearity among allogamous 
      red clover varieties. This integration of genetic linkage and quantitative 
      chromosome maps should provide valuable insight into allogamous legume genetics.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - Kataoka, R
AU  - Kataoka R
AD  - Graduate School of Human Development and Environment, Kobe University, Kobe, 
      Japan.
FAU - Hara, M
AU  - Hara M
FAU - Kato, S
AU  - Kato S
FAU - Isobe, S
AU  - Isobe S
FAU - Sato, S
AU  - Sato S
FAU - Tabata, S
AU  - Tabata S
FAU - Ohmido, N
AU  - Ohmido N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120711
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - *Chromosome Mapping
MH  - Chromosomes, Artificial, Bacterial
MH  - *Chromosomes, Plant
MH  - *Genetic Linkage
MH  - In Situ Hybridization, Fluorescence
MH  - Telomere
MH  - Trifolium/*genetics
EDAT- 2012/07/17 06:00
MHDA- 2012/10/16 06:00
CRDT- 2012/07/17 06:00
PHST- 2012/03/28 00:00 [accepted]
PHST- 2012/07/17 06:00 [entrez]
PHST- 2012/07/17 06:00 [pubmed]
PHST- 2012/10/16 06:00 [medline]
AID - 000339509 [pii]
AID - 10.1159/000339509 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2012;137(1):60-9. doi: 10.1159/000339509. Epub 2012 Jul 11.