PMID- 22799198
OWN - NLM
STAT- MEDLINE
DCOM- 20140702
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 4
DP  - 2012 Apr
TI  - [Determination of mannose-6-phosphate in pharmaceutical intermediates by high 
      performance ion-exchange chromatography-electrochemical detection].
PG  - 395-9
AB  - A novel method was developed for the determination of mannose-6-phosphate and the 
      phosphate in pharmaceutical intermediates by high performance ion-exchange 
      chromatography (IC) and electrochemical detection. The sample was dissolved with 
      purified water, filtrated by a 0.22 microm nylon filter membrane, and then 
      separated by ion-exchange chromatography. The separation was performed on an 
      IonPac AS18 column (250 mm x4 mm) with an IonPac AG18 (50 mm x4 mm) as guard 
      column, and 25 mmol/L KOH solution as the eluent at a flow rate of 1.0 mL/min. 
      The detection was performed with electrochemical detectors (an integrated pulsed 
      amperometric detector and a suppressed conductivity detector in line). 
      Mannose-6-phosphate was selectively determined with an integrated pulsed 
      amperometric detector first. Then, both of mannose-6-phosphate and phosphate were 
      determined with a suppressed conductivity detector after the background 
      conductance of KOH was suppressed with the ASRS suppressor. The injection volume 
      was 25 microL. The external standard calibration curve was used for quantitative 
      analysis. With an amperometric detector, the linear range of the method for 
      mannose-6-phosphate were 0.06 - 10.0 mg/L (r = 0.9998). The recoveries were 92.1% 
      - 103.1% with the relative standard deviations (RSDs) less than 3%. The detection 
      limit was 0.02 mg/L for mannose-6-phosphate. The method is simple, effective, 
      sensitive and selective. It can be used to determine the quality of 
      pharmaceutical intermediates.
FAU - Pan, Guangwen
AU  - Pan G
AD  - Shanghai Lab., Thermo Fisher Scientific Inc., Shanghai 201203, China. 
      arpanny@163.com
FAU - Liu, Luye
AU  - Liu L
FAU - Ye, Mingli
AU  - Ye M
FAU - Hu, Zhongyang
AU  - Hu Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Mannosephosphates)
RN  - 0 (Pharmaceutical Preparations)
RN  - 0 (Phosphates)
RN  - 3672-15-9 (mannose-6-phosphate)
SB  - IM
MH  - *Chromatography, Ion Exchange
MH  - *Electrochemical Techniques
MH  - Mannosephosphates/*analysis/isolation & purification
MH  - Pharmaceutical Preparations/analysis
MH  - Phosphates/*analysis/isolation & purification
EDAT- 2012/07/18 06:00
MHDA- 2014/07/06 06:00
CRDT- 2012/07/18 06:00
PHST- 2012/07/18 06:00 [entrez]
PHST- 2012/07/18 06:00 [pubmed]
PHST- 2014/07/06 06:00 [medline]
AID - 10.3724/sp.j.1123.2011.12061 [doi]
PST - ppublish
SO  - Se Pu. 2012 Apr;30(4):395-9. doi: 10.3724/sp.j.1123.2011.12061.