PMID- 22800573
OWN - NLM
STAT- MEDLINE
DCOM- 20130131
LR  - 20181201
IS  - 1873-4995 (Electronic)
IS  - 0168-3659 (Linking)
VI  - 162
IP  - 2
DP  - 2012 Sep 10
TI  - Prolonged gene silencing by siRNA/chitosan-g-deoxycholic acid polyplexes loaded 
      within biodegradable polymer nanoparticles.
PG  - 407-13
LID - 10.1016/j.jconrel.2012.07.006 [doi]
AB  - Recently, small interfering RNA (siRNA) has received much attention for 
      therapeutic applications; however, low transfection efficiency and intrinsic 
      instability limit effective gene silencing. Here we show a new approach based on 
      the incorporation of siRNA/polyelectrolyte complexes into biodegradable 
      poly(d,l-lactic-co-glycolic acid) (PLGA) nanoparticles to stabilize siRNA within 
      a hydrophobic solid matrix for prolonged gene silencing. To solubilize siRNA in 
      organic media, chitosan oligosaccharides grafted with deoxycholic acids are 
      synthesized and complexed with siRNA, generating a self-assembled polyelectrolyte 
      complex of 123.9 +/- 56.8 nm in diameter. The complex is mixed with PLGA solution 
      and emulsified in water to prepare siRNA-loaded PLGA nanoparticles having a 
      diameter of about 230 nm. The excellent structural stability of the prepared 
      nanoparticles leads to efficient cellular uptake followed by effective gene 
      silencing even in the presence of serum proteins. These results suggest that the 
      encapsulation of siRNA into biodegradable polymer matrix can be an effective 
      means of improving the structural stability of siRNA for prolonged therapeutic 
      efficacy.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Lee, Jeong Yu
AU  - Lee JY
AD  - Department of Materials Science and Engineering, Korea Advanced Institute of 
      Science and Technology, Daejeon 305-701, Republic of Korea.
FAU - Lee, Soo Hyeon
AU  - Lee SH
FAU - Oh, Mi Hwa
AU  - Oh MH
FAU - Kim, Jee Seon
AU  - Kim JS
FAU - Park, Tae Gwan
AU  - Park TG
FAU - Nam, Yoon Sung
AU  - Nam YS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120716
PL  - Netherlands
TA  - J Control Release
JT  - Journal of controlled release : official journal of the Controlled Release 
      Society
JID - 8607908
RN  - 0 (Oligosaccharides)
RN  - 0 (RNA, Small Interfering)
RN  - 005990WHZZ (Deoxycholic Acid)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Biological Transport
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Chitosan/*administration & dosage/chemistry
MH  - Deoxycholic Acid/*administration & dosage/chemistry
MH  - Gene Silencing
MH  - Green Fluorescent Proteins/genetics
MH  - Humans
MH  - Lactic Acid/*administration & dosage/chemistry
MH  - Nanoparticles/administration & dosage/chemistry
MH  - Oligosaccharides/*administration & dosage/chemistry
MH  - Polyglycolic Acid/*administration & dosage/chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - RNA, Small Interfering/*administration & dosage/genetics
MH  - Transfection
EDAT- 2012/07/18 06:00
MHDA- 2013/02/01 06:00
CRDT- 2012/07/18 06:00
PHST- 2012/03/28 00:00 [received]
PHST- 2012/06/14 00:00 [revised]
PHST- 2012/07/04 00:00 [accepted]
PHST- 2012/07/18 06:00 [entrez]
PHST- 2012/07/18 06:00 [pubmed]
PHST- 2013/02/01 06:00 [medline]
AID - S0168-3659(12)00546-9 [pii]
AID - 10.1016/j.jconrel.2012.07.006 [doi]
PST - ppublish
SO  - J Control Release. 2012 Sep 10;162(2):407-13. doi: 10.1016/j.jconrel.2012.07.006. 
      Epub 2012 Jul 16.