PMID- 22800829
OWN - NLM
STAT- MEDLINE
DCOM- 20121207
LR  - 20120717
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 125
IP  - 9
DP  - 2012 May
TI  - Overexpression of interleukin-l7 in tumor-associated macrophages is correlated 
      with the differentiation and angiogenesis of laryngeal squamous cell carcinoma.
PG  - 1603-7
AB  - BACKGROUND: Interleukin-l7 (IL-17), which exerts strong pro-inflammatory effects, 
      has emerged as an important mediator in inflammation-associated cancer. The aim 
      of this study was to clarify the relationship between IL-17 and tumor associated 
      macrophages (TAMs), and the correlation of the microvessel density in the 
      development of laryngeal squamous cell carcinoma (LSCC). METHODS: 
      Histopathological observations and immunohistochemistry staining for IL-17, CD68, 
      and CD34 were performed on 72 specimens (32 cases of LSCC, 20 cases of adjacent 
      tissues of carcinoma as controls, and 20 cases of chronic hypertrophic 
      laryngitis). Double immunohistochemical staining was done to determine which 
      cells expressed IL-17. Real-time quantitative PCR determined the mRNA expression 
      of IL-17. ELISA was used to detect the expression of the serum level of IL-17 in 
      the three groups. RESULTS: The inflammation response had increased in LSCC. 
      Overexpression of IL-17 and CD68 protein were seen in LSCC (P < 0.01). The 
      expression of IL-17 was different between well and poorly differentiated LSCC (P 
      < 0.01). The IL-17 expressing cells were mainly located in macrophages 
      (CD68(+)/IL17(+)) as demonstrated by double immunohistochemical staining. IL-17 
      expression significantly correlated with high microvessel density (CD34(+)) in 
      LSCC (P < 0.05). Relatively higher mRNA expression levels of IL-17 were seen in 
      LSCC compared to the controls (P < 0.05). The serum expression of IL-17 was 
      similar among the three groups (P > 0.05). CONCLUSION: IL-17 was expressed by 
      TAMs, and IL-17 may significantly correlate to the differentiation and 
      angiogenesis in the development of LSCC.
FAU - Meng, Cui-da
AU  - Meng CD
AD  - Department of Otorhinolaryngology, Head & Neck Surgery, China-Japan Union 
      Hospital, Jilin University, Changchun, Jilin 130033, China.
FAU - Zhu, Dong-Dong
AU  - Zhu DD
FAU - Jiang, Xiao-Dan
AU  - Jiang XD
FAU - Li, Lin
AU  - Li L
FAU - Sha, Ji-Chao
AU  - Sha JC
FAU - Dong, Zhen
AU  - Dong Z
FAU - Kong, Hong
AU  - Kong H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Interleukin-17)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/genetics/*metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-17/genetics/*metabolism
MH  - Laryngeal Neoplasms/genetics/*metabolism
MH  - Macrophages/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Neovascularization, Pathologic/genetics/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Young Adult
EDAT- 2012/07/18 06:00
MHDA- 2012/12/12 06:00
CRDT- 2012/07/18 06:00
PHST- 2012/07/18 06:00 [entrez]
PHST- 2012/07/18 06:00 [pubmed]
PHST- 2012/12/12 06:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2012 May;125(9):1603-7.