PMID- 22801546
OWN - NLM
STAT- MEDLINE
DCOM- 20130201
LR  - 20211021
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 11
IP  - 15
DP  - 2012 Aug 1
TI  - Tetraploid cells from cytokinesis failure induce aneuploidy and spontaneous 
      transformation of mouse ovarian surface epithelial cells.
PG  - 2864-75
LID - 10.4161/cc.21196 [doi]
AB  - Most ovarian cancers originate from the ovarian surface epithelium and are 
      characterized by aneuploid karyotypes. Aneuploidy, a consequence of chromosome 
      instability, is an early event during the development of ovarian cancers. 
      However, how aneuploid cells are evolved from normal diploid cells in ovarian 
      cancers remains unknown. In the present study, cytogenetic analyses of a mouse 
      syngeneic ovarian cancer model revealed that diploid mouse ovarian surface 
      epithelial cells (MOSECs) experienced an intermediate tetraploid cell stage, 
      before evolving to aneuploid (mainly near-tetraploid) cells. Using long-term 
      live-cell imaging followed by fluorescence in situ hybridization (FISH), we 
      demonstrated that tetraploid cells originally arose from cytokinesis failure of 
      bipolar mitosis in diploid cells, and gave rise to aneuploid cells through 
      chromosome mis-segregation during both bipolar and multipolar mitoses. Injection 
      of the late passage aneuploid MOSECs resulted in tumor formation in C57BL/6 mice. 
      Therefore, we reveal a pathway for the evolution of diploid to aneuploid MOSECs 
      and elucidate a mechanism for the development of near-tetraploid ovarian cancer 
      cells.
FAU - Lv, Lei
AU  - Lv L
AD  - Hefei National Laboratory for Physical Sciences at Microscale and School of Life 
      Sciences, University of Science and Technology of China, Hefei, China.
FAU - Zhang, Tianwei
AU  - Zhang T
FAU - Yi, Qiyi
AU  - Yi Q
FAU - Huang, Yun
AU  - Huang Y
FAU - Wang, Zheng
AU  - Wang Z
FAU - Hou, Heli
AU  - Hou H
FAU - Zhang, Huan
AU  - Zhang H
FAU - Zheng, Wei
AU  - Zheng W
FAU - Hao, Qiaomei
AU  - Hao Q
FAU - Guo, Zongyou
AU  - Guo Z
FAU - Cooke, Howard J
AU  - Cooke HJ
FAU - Shi, Qinghua
AU  - Shi Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120801
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
SB  - IM
CIN - Cell Cycle. 2012 Sep 1;11(17):3157-8. PMID: 22895170
CIN - Cell Cycle. 2012 Sep 15;11(18):3354-5. PMID: 22918235
MH  - *Aneuploidy
MH  - Animals
MH  - Cell Line
MH  - *Cell Transformation, Neoplastic/genetics
MH  - *Chromosome Segregation
MH  - *Cytokinesis
MH  - Epithelial Cells
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitosis
MH  - Ovarian Neoplasms/genetics/metabolism/*pathology
MH  - Ovary/metabolism/*pathology
MH  - *Tetraploidy
PMC - PMC3419060
EDAT- 2012/07/18 06:00
MHDA- 2013/02/05 06:00
PMCR- 2012/08/01
CRDT- 2012/07/18 06:00
PHST- 2012/07/18 06:00 [entrez]
PHST- 2012/07/18 06:00 [pubmed]
PHST- 2013/02/05 06:00 [medline]
PHST- 2012/08/01 00:00 [pmc-release]
AID - 21196 [pii]
AID - 2012CC4173R [pii]
AID - 10.4161/cc.21196 [doi]
PST - ppublish
SO  - Cell Cycle. 2012 Aug 1;11(15):2864-75. doi: 10.4161/cc.21196. Epub 2012 Aug 1.