PMID- 22803352
OWN - NLM
STAT- MEDLINE
DCOM- 20120816
LR  - 20120718
IS  - 0001-6209 (Print)
IS  - 0001-6209 (Linking)
VI  - 52
IP  - 5
DP  - 2012 May 4
TI  - [Recombinant porcine lung surfactant protein A inhibits porcine reproductive and 
      respiratory syndrome virus infection into host cells in vitro].
PG  - 654-60
AB  - OBJECTIVE: To investigate the antiviral activity of porcine lung surfactant 
      protein A (SP-A) to porcine reproductive and respiratory syndrome virus (PRRSV) 
      in vitro. METHODS: The SP-A gene was amplified by PCR from the plasmid containing 
      porcine SP-A gene, and subcloned into pcDNA3. 1A-CD5 vector containing the human 
      CD5 signal peptide to generate SP-A eukaryotic expression vector 
      pcDNA-CD5-SPA/MH. The recombinant expression vector was transfected into HEK293T 
      cells mediated with calcium phosphate. The expressed recombinant SP-A was 
      identified by Western blot and purified from culture medium by Ni-NTA-Agarose 
      beads. The binding activity of SP-A with PRRSV was identified by ELISA. The 
      antiviral activity of SP-A to PRRSV was analyzed by viral titer reduction assays 
      on MARC-145 cells and porcine alveolar macrophages (PAM). RESULTS: The results 
      showed that the eukaryotic expression vector of SP-A gene could mediate SP-A 
      expression in HEK293T cells, the expressed SP-A could bind PRRSV in a dose 
      dependent manner. The PRRSV incubated in advance with SP-A showed the lower 
      infective activity compared with no-SP-A-incubated PRRSV on both MARC-145 cells 
      and porcine alveolar macrophages. The SP-A-treated PRRSV titers in MARC-145 cells 
      and PAM cells were significantly lower than that of SP-A-untreated PRRSV at 72 h 
      post-infection. CONCLUSION: Recombinant porcine SP-A significantly inhibit the 
      infection of PRRSV to the host cells in vitro, which indicates that recombinant 
      SP-A possesses anti-PRRSV activity.
FAU - Zhang, Feng
AU  - Zhang F
AD  - Department of Basic Veterinary Medicine, College of Veterinary Medicine, 
      Agricultural University of Hebei, North China Research Center of Animal Epidemic 
      Pathogen Biology, Ministry of Agriculture of China, Baoding 071001, China. 
      zhangfeng2084@163.com
FAU - Zhong, Fei
AU  - Zhong F
FAU - Li, Xiujin
AU  - Li X
FAU - Wang, Xingxing
AU  - Wang X
FAU - Zhang, Kao
AU  - Zhang K
FAU - Chen, Huihui
AU  - Chen H
FAU - Li, Zhen
AU  - Li Z
FAU - Li, Wenyan
AU  - Li W
FAU - Pan, Hongli
AU  - Pan H
FAU - Han, Dongmei
AU  - Han D
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Wei Sheng Wu Xue Bao
JT  - Wei sheng wu xue bao = Acta microbiologica Sinica
JID - 21610860R
RN  - 0 (Pulmonary Surfactant-Associated Protein A)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - HEK293 Cells
MH  - Humans
MH  - Macrophages, Alveolar/immunology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/immunology/*physiology
MH  - Pulmonary Surfactant-Associated Protein A/biosynthesis/genetics/*immunology
MH  - Recombinant Proteins/biosynthesis/genetics/immunology
MH  - Swine
MH  - Virus Replication
EDAT- 2012/07/19 06:00
MHDA- 2012/08/17 06:00
CRDT- 2012/07/19 06:00
PHST- 2012/07/19 06:00 [entrez]
PHST- 2012/07/19 06:00 [pubmed]
PHST- 2012/08/17 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Wu Xue Bao. 2012 May 4;52(5):654-60.