PMID- 22805279
OWN - NLM
STAT- MEDLINE
DCOM- 20140211
LR  - 20211021
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 16
IP  - 12
DP  - 2012 Dec
TI  - Cell fusion contributes to the rescue of apoptotic cardiomyocytes by bone marrow 
      cells.
PG  - 3085-95
LID - 10.1111/j.1582-4934.2012.01600.x [doi]
AB  - Cardiomyocyte apoptosis is an important contributor to the progressive cardiac 
      dysfunction that culminates in congestive heart failure. Bone marrow cells (BMCs) 
      restore cardiac function following ischaemia, and transplanted BMCs have been 
      reported to fuse with cells of diverse tissues. We previously demonstrated that 
      the myogenic conversion of bone marrow stromal cells increased nearly twofold 
      when the cells were co-cultured with apoptotic (TNF-alpha treated) cardiomyocytes. We 
      therefore hypothesized that cell fusion may be a major mechanism by which BMCs 
      rescue cardiomyocytes from apoptosis. We induced cellular apoptosis in neonatal 
      rat cardiomyocytes by treatment with hydrogen peroxide (H(2)O(2)). The TUNEL 
      assay demonstrated an increase in apoptosis from 4.5 +/- 1.3% in non-treated cells 
      to 19.0 +/- 4.4% (P < 0.05) in treated cells. We subsequently co-cultured the 
      apoptotic cardiomyocytes with BMCs and assessed cell fusion using flow cytometry. 
      Fusion was rare in the non-treated control cardiomyocytes (0.3%), whereas 
      H(2)O(2) treatment led to significantly higher fusion rates than the control 
      group (P < 0.05), with the highest rate of 7.9 +/- 0.3% occurring at 25 muM 
      H(2)O(2). We found an inverse correlation between cell fusion and completion of 
      cardiomyocyte apoptosis (R(2) = 0.9863). An in vivo mouse model provided evidence 
      of cell fusion in the infarcted myocardium following the injection of BMCs. The 
      percentage of cells undergoing fusion was significantly higher in mice injected 
      with BMCs following infarction (8.8 +/- 1.3%) compared to mice that did not undergo 
      infarction (4.6 +/- 0.6%, P < 0.05). Enhancing cell fusion may be one method to 
      preserve cardiomyocytes following myocardial infarction, and this new approach 
      may provide a novel target for cardiac regenerative therapies.
CI  - (c) 2012 The Authors Journal of Cellular and Molecular Medicine (c) 2012 Foundation 
      for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
FAU - Yang, Wei-Jian
AU  - Yang WJ
AD  - Department of Cardiology, Second Affiliated Hospital of Guangzhou Medical 
      College, Guangzhou, China.
FAU - Li, Shu-Hong
AU  - Li SH
FAU - Weisel, Richard D
AU  - Weisel RD
FAU - Liu, Shi-Ming
AU  - Liu SM
FAU - Li, Ren-Ke
AU  - Li RK
LA  - eng
GR  - MOP102535/Canadian Institutes of Health Research/Canada
GR  - MOP86661/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Bone Marrow Cells/*physiology
MH  - *Cell Fusion
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Green Fluorescent Proteins
MH  - Heart Failure/*physiopathology
MH  - Hematopoietic Stem Cells/physiology
MH  - Hydrogen Peroxide/pharmacology
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Myocardial Infarction/*physiopathology
MH  - Myocytes, Cardiac/*physiology
MH  - Oxidative Stress
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
PMC - PMC4393736
EDAT- 2012/07/19 06:00
MHDA- 2014/02/12 06:00
PMCR- 2012/12/01
CRDT- 2012/07/19 06:00
PHST- 2011/12/20 00:00 [received]
PHST- 2012/07/09 00:00 [accepted]
PHST- 2012/07/19 06:00 [entrez]
PHST- 2012/07/19 06:00 [pubmed]
PHST- 2014/02/12 06:00 [medline]
PHST- 2012/12/01 00:00 [pmc-release]
AID - 10.1111/j.1582-4934.2012.01600.x [doi]
PST - ppublish
SO  - J Cell Mol Med. 2012 Dec;16(12):3085-95. doi: 10.1111/j.1582-4934.2012.01600.x.