PMID- 22805836
OWN - NLM
STAT- MEDLINE
DCOM- 20130212
LR  - 20221207
IS  - 1573-0972 (Electronic)
IS  - 0959-3993 (Linking)
VI  - 28
IP  - 3
DP  - 2012 Mar
TI  - Isolation, characterization and colonization of 1-aminocyclopropane-1-carboxylate 
      deaminase-producing bacteria XG32 and DP24.
PG  - 1155-62
LID - 10.1007/s11274-011-0918-9 [doi]
AB  - Two 1-aminocyclopropane-1-carboxylate deaminase-producing bacterial strains (DP24 
      and XG32) were isolated from surface-sterilized tomato roots and rizhospere soil. 
      The strains were identified as Pseudomonas fluorescens biovar. IV (XG2) and 
      Erwinia herbicola (DP24) by physiological and biochemical tests, and 16S rRNA 
      gene analysis. Both strains showed positive plant growth-promoting activity when 
      inoculated into cucumber (Cucumis sativus), tomato (Lycopersicon esculentum), 
      pepper (Capsicum annuum) and rapeseed (Brassica napus L.). Colonization ability 
      and behavior of these two strains were determined by treating mutant strains with 
      rifampicin and fluorescence in situ hybridization (FISH) assay with rRNA targeted 
      probes, respectively. Both strains were endophytic colonizers of pepper plants. 
      The behavior of the two strains was not identical. Strain XG32 only colonized the 
      root and reached the max level of 27.7 x 10(7) c.f.u./g (fresh weight), after 12 
      days postinoculation, while strain DP24 was able to colonize the roots, stems and 
      leaves. The max level was reached at 40.87 x 10(7) c.f.u./g (fresh weight) in the 
      roots, 17 x 10(7) c.f.u./g in the stems after 7 days postinoculation and 44.84 x 
      10(7) c.f.u./g in the leaves after 12 days postinoculation.
FAU - Wang, Mei-Xia
AU  - Wang MX
AD  - Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life 
      Sciences, Nanjing Normal University, Nanjing 210046, China.
FAU - Liu, Jia
AU  - Liu J
FAU - Chen, Shuang-Lin
AU  - Chen SL
FAU - Yan, Shu-Zhen
AU  - Yan SZ
LA  - eng
SI  - GENBANK/DQ431467
SI  - GENBANK/HM235925
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111022
PL  - Germany
TA  - World J Microbiol Biotechnol
JT  - World journal of microbiology & biotechnology
JID - 9012472
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - EC 3.5.99.7 (1-aminocyclopropane-1-carboxylate deaminase)
RN  - EC 4.1.- (Carbon-Carbon Lyases)
SB  - IM
MH  - Bacterial Load
MH  - Bacterial Typing Techniques
MH  - Brassica napus/growth & development
MH  - Capsicum/growth & development
MH  - Carbon-Carbon Lyases/*biosynthesis/*genetics
MH  - Cluster Analysis
MH  - Cucumis sativus/growth & development
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Endophytes/classification/enzymology/genetics/isolation & purification
MH  - Erwinia/classification/*enzymology/genetics/*isolation & purification
MH  - Solanum lycopersicum/growth & development/microbiology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Plant Roots/microbiology
MH  - Pseudomonas fluorescens/classification/*enzymology/genetics/*isolation & 
      purification
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rhizosphere
MH  - Sequence Analysis, DNA
MH  - Soil Microbiology
EDAT- 2012/07/19 06:00
MHDA- 2013/02/13 06:00
CRDT- 2012/07/19 06:00
PHST- 2011/07/13 00:00 [received]
PHST- 2011/10/04 00:00 [accepted]
PHST- 2012/07/19 06:00 [entrez]
PHST- 2012/07/19 06:00 [pubmed]
PHST- 2013/02/13 06:00 [medline]
AID - 10.1007/s11274-011-0918-9 [doi]
PST - ppublish
SO  - World J Microbiol Biotechnol. 2012 Mar;28(3):1155-62. doi: 
      10.1007/s11274-011-0918-9. Epub 2011 Oct 22.