PMID- 22806454
OWN - NLM
STAT- MEDLINE
DCOM- 20130328
LR  - 20211203
IS  - 1521-4141 (Electronic)
IS  - 0014-2980 (Linking)
VI  - 42
IP  - 11
DP  - 2012 Nov
TI  - The CD20 homolog Ms4a8a integrates pro- and anti-inflammatory signals in novel 
      M2-like macrophages and is expressed in parasite infection.
PG  - 2971-82
LID - 10.1002/eji.201142331 [doi]
AB  - Recently, we identified the CD20 homolog Ms4a8a as a novel molecule expressed by 
      tumor-associated macrophages that directly enhances tumor growth. Here, we 
      analyzed Ms4a8a(+) macrophages in M2-associated infectious pathologies. In 
      late-stage Trypanosoma congolense and Taenia crassiceps infections, Ms4a8a 
      expression was detected in hepatic and peritoneal macrophages respectively. 
      Innate immunity in these infections is modulated by Toll-like receptor (TLR) 
      signaling and TLR2/4/7 agonists strongly induced Ms4a8a expression in bone marrow 
      derived macrophages (BMDMs) treated with M2 mediators (glucocorticoids/IL-4). 
      LPS/dexamethasone/IL-4-induced Ms4a8a(+) BMDMs were characterized by strong 
      expression of mRNA of mannose receptor (Mmr), arginase 1, and CD163, and by 
      decreased iNOS expression. Coinduction of Ms4a8a by M2 mediators and TLR agonists 
      involved the classical TLR signaling cascade via activation of MyD88/TRIF and 
      NF-kappaB. Forced overexpression of Ms4a8a modulated the TLR4 response of RAW264.7 
      cells as shown by gene expression profiling. Upregulation of Hdc, Tcfec, and Sla 
      was confirmed both in primary LPS/dexamethasone/IL-4-stimulated Ms4a8a(+) BMDMs 
      and in peritoneal macrophages from late-stage Taenia crassiceps infection. In 
      conclusion, we show that TLR signaling skews the typical alternative macrophage 
      activation program to induce a special M2-like macrophage subset in vitro that 
      also occurs in immunomodulatory immune reactions in vivo, a process directly 
      involving the CD20 homolog Ms4a8a.
CI  - (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Schmieder, Astrid
AU  - Schmieder A
AD  - Department of Dermatology, Venereology and Allergology, University Medical Center 
      and Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany. 
      astrid.schmieder@umm.de
FAU - Schledzewski, Kai
AU  - Schledzewski K
FAU - Michel, Julia
AU  - Michel J
FAU - Schonhaar, Kathrin
AU  - Schonhaar K
FAU - Morias, Yannick
AU  - Morias Y
FAU - Bosschaerts, Tom
AU  - Bosschaerts T
FAU - Van den Bossche, Jan
AU  - Van den Bossche J
FAU - Dorny, Pierre
AU  - Dorny P
FAU - Sauer, Andrea
AU  - Sauer A
FAU - Sticht, Carsten
AU  - Sticht C
FAU - Geraud, Cyrill
AU  - Geraud C
FAU - Waibler, Zoe
AU  - Waibler Z
FAU - Beschin, Alain
AU  - Beschin A
FAU - Goerdt, Sergij
AU  - Goerdt S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120905
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD20)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Mannose Receptor)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Toll-Like Receptors)
RN  - 63231-63-0 (RNA)
RN  - EC 3.5.3.1 (Arg1 protein, mouse)
RN  - EC 3.5.3.1 (Arginase)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/immunology
MH  - Antigens, CD20/*immunology
MH  - Antigens, Differentiation, Myelomonocytic/genetics/immunology
MH  - Arginase/genetics/immunology
MH  - Cell Line
MH  - Immunity, Innate/immunology
MH  - Lectins, C-Type/genetics/immunology
MH  - Macrophage Activation/immunology
MH  - Macrophages/*immunology/parasitology
MH  - Mannose Receptor
MH  - Mannose-Binding Lectins/genetics/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA/chemistry/genetics
MH  - RNA, Messenger/chemistry/genetics
MH  - Receptors, Cell Surface/genetics/immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/immunology
MH  - Specific Pathogen-Free Organisms
MH  - Taenia/*immunology
MH  - Taeniasis/*immunology/parasitology
MH  - Toll-Like Receptors/agonists
MH  - Trypanosoma congolense/*immunology
MH  - Trypanosomiasis, African/*immunology/parasitology
EDAT- 2012/07/19 06:00
MHDA- 2013/03/30 06:00
CRDT- 2012/07/19 06:00
PHST- 2011/12/14 00:00 [received]
PHST- 2012/06/14 00:00 [revised]
PHST- 2012/07/10 00:00 [accepted]
PHST- 2012/07/19 06:00 [entrez]
PHST- 2012/07/19 06:00 [pubmed]
PHST- 2013/03/30 06:00 [medline]
AID - 10.1002/eji.201142331 [doi]
PST - ppublish
SO  - Eur J Immunol. 2012 Nov;42(11):2971-82. doi: 10.1002/eji.201142331. Epub 2012 Sep 
      5.