PMID- 22806472
OWN - NLM
STAT- MEDLINE
DCOM- 20121106
LR  - 20120718
IS  - 1522-2683 (Electronic)
IS  - 0173-0835 (Linking)
VI  - 33
IP  - 13
DP  - 2012 Jul
TI  - Micellar nanotubes dispersed electrokinetic chromatography for the simultaneous 
      determination of antibiotics in bovine milk.
PG  - 2049-55
LID - 10.1002/elps.201100713 [doi]
AB  - A method to determine four antibiotics for veterinary use (ciprofloxacin, 
      enrofloxacin, florfenicol, and chloramphenicol) of different families 
      (fluoroquinolones and amphenicols) in bovine milk was developed. The 
      determination of the analytes was carried out using micellar electrokinetic 
      capillary chromatography (MEKC) with a common sodium borate-SDS buffer solution 
      containing single-walled carbon nanotubes (SWCNTs). In this way, a great 
      improvement in the electrophoretic resolution and the separation efficiency was 
      achieved compared to MEKC. An online reverse electrode polarity-stacking mode 
      (REPSM) was carried out to enhance sensitivity. This step was performed in only 2 
      min and it allowed a stacked percentage of 103. That means that all the amount of 
      injected analytes is effectively stacked. When this stacking procedure was 
      combined with an off-line preconcentration step, based on SPE, analytes could be 
      detected in lower concentration than the established maximum residue limits 
      (MRLs). The LODs for the four compounds were between 6.8 and 13.8 mug L(-1) and 
      the RSD values were between 1.1% and 6.6%. The whole method was applied to spiked 
      real samples with acceptable precision and satisfactory recoveries.
CI  - (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Springer, Valeria H
AU  - Springer VH
AD  - FIA Laboratory, Analytical Chemistry Section, INQUISUR (UNS-CONICET), Bahia 
      Blanca, Buenos Aires, Argentina.
FAU - Lista, Adriana G
AU  - Lista AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Nanotubes, Carbon)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis/isolation & purification
MH  - Cattle
MH  - Chromatography, Micellar Electrokinetic Capillary/*instrumentation/*methods
MH  - Drug Residues/*analysis/isolation & purification
MH  - Limit of Detection
MH  - Milk/*chemistry
MH  - Nanotubes, Carbon/*chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solid Phase Extraction
EDAT- 2012/07/19 06:00
MHDA- 2012/11/07 06:00
CRDT- 2012/07/19 06:00
PHST- 2012/07/19 06:00 [entrez]
PHST- 2012/07/19 06:00 [pubmed]
PHST- 2012/11/07 06:00 [medline]
AID - 10.1002/elps.201100713 [doi]
PST - ppublish
SO  - Electrophoresis. 2012 Jul;33(13):2049-55. doi: 10.1002/elps.201100713.