PMID- 22806643
OWN - NLM
STAT- MEDLINE
DCOM- 20130415
LR  - 20211021
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 90
IP  - 11
DP  - 2012 Nov
TI  - Protective role of mu opioid receptor activation in intestinal inflammation 
      induced by mesenteric ischemia/reperfusion in mice.
PG  - 2146-53
LID - 10.1002/jnr.23108 [doi]
AB  - Intestinal ischemia is a clinical emergency with high morbidity and mortality. We 
      investigated whether activation of mu opioid receptor (muOR) protects from the 
      inflammation induced by intestinal ischemia and reperfusion (I/R) in mice. 
      Ischemia was induced by occlusion of the superior mesenteric artery (45 min), 
      followed by reperfusion (5 hr). Sham-operated (SO) and normal (N) mice served as 
      controls. Each group received subcutaneously 1) saline solution, 2) the muOR 
      selective agonist [D-Ala2, N-Me-Phe4, Gly5-ol]-enkephalin (DAMGO; 0.01 mg kg(-1) 
      ), 3) DAMGO and the selective muOR antagonist 
      [H-D-Phe-Cys-Tyr-D-Trp-Arg-Thr-Pen-Thr-NH2] (CTAP; 0.1 mg kg(-1) ), or 4) CTAP 
      alone. I/R induced intestinal inflammation as indicated by histological damage 
      and the significant increase in myeloperoxidase (MPO) activity, an index of 
      tissue neutrophil accumulation. Tumor necrosis factor-alpha (TNF-alpha) and 
      interleukin-10 (IL-10) mRNA levels were also increased in I/R mice compared with 
      SO. DAMGO significantly reduced tissue damage, MPO activity, and TNF-alpha mRNA 
      levels in I/R, and these effects were reversed by CTAP. By contrast, DAMGO did 
      not modify IL-10 mRNA levels or gastrointestinal transit. DAMGO's effects are 
      receptor mediated and likely are due to activation of peripheral muORs, because it 
      does not readily cross the blood-brain barrier. These findings suggest that 
      activation of peripheral muOR protects from the inflammatory response induced by 
      I/R through a pathway involving the proinflammatory cytokine TNF-alpha. Reduction of 
      acute inflammation might prevent I/R complications, including motility 
      impairment, which develop at a later stage of reperfusion and likely are due to 
      inflammatory cell infiltrates.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Saccani, Francesca
AU  - Saccani F
AD  - Department of Medicine, David Geffen School of Medicine at the University of 
      California, Los Angeles, Los Angeles, California, USA.
FAU - Anselmi, Laura
AU  - Anselmi L
FAU - Jaramillo, Ingrid
AU  - Jaramillo I
FAU - Bertoni, Simona
AU  - Bertoni S
FAU - Barocelli, Elisabetta
AU  - Barocelli E
FAU - Sternini, Catia
AU  - Sternini C
LA  - eng
GR  - DK41301/DK/NIDDK NIH HHS/United States
GR  - R56 DK054155/DK/NIDDK NIH HHS/United States
GR  - P30 DK041301/DK/NIDDK NIH HHS/United States
GR  - DK54155/DK/NIDDK NIH HHS/United States
GR  - R01 DK054155/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120717
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Cytokines)
RN  - 0 (Receptors, Opioid, mu)
SB  - IM
MH  - Animals
MH  - Cytokines/biosynthesis
MH  - Female
MH  - Inflammation/etiology/*metabolism/pathology
MH  - Intestinal Mucosa/*metabolism
MH  - Intestines/blood supply/pathology
MH  - Mesentery/blood supply
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, Opioid, mu/*metabolism
MH  - Reperfusion Injury/complications/*metabolism/pathology
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC3439549
MID - NIHMS384620
EDAT- 2012/07/19 06:00
MHDA- 2013/04/16 06:00
PMCR- 2013/11/01
CRDT- 2012/07/19 06:00
PHST- 2011/09/20 00:00 [received]
PHST- 2012/05/27 00:00 [revised]
PHST- 2012/05/31 00:00 [accepted]
PHST- 2012/07/19 06:00 [entrez]
PHST- 2012/07/19 06:00 [pubmed]
PHST- 2013/04/16 06:00 [medline]
PHST- 2013/11/01 00:00 [pmc-release]
AID - 10.1002/jnr.23108 [doi]
PST - ppublish
SO  - J Neurosci Res. 2012 Nov;90(11):2146-53. doi: 10.1002/jnr.23108. Epub 2012 Jul 
      17.