PMID- 22806912
OWN - NLM
STAT- MEDLINE
DCOM- 20121113
LR  - 20220408
IS  - 1699-5848 (Electronic)
IS  - 0213-3911 (Linking)
VI  - 27
IP  - 9
DP  - 2012 Sep
TI  - In situ hybridization studies favouring the occurrence of a local production of 
      BDNF in the human Achilles tendon.
PG  - 1239-46
LID - 10.14670/HH-27.1239 [doi]
AB  - Brain derived neurotrophic factor (BDNF) is a multipotent neurotrophin known for 
      its growth-influencing and apoptosis-modulating functions, as well as for its 
      function to interact with neurotransmitters/neuromodulators. BDNF is reported to 
      be mainly produced in the brain. BDNF can be absorbed into peripheral tissue from 
      the blood stream. Expression of this neurotrophin at the protein level, as well 
      as of the neurotrophin receptor p75, has been previously shown for the principal 
      cells (tenocytes) of the Achilles tendon. However, there is no proof at the mRNA 
      level that BDNF is produced by the tenocytes. As the Achilles tendon tenocytes 
      show "neuronal-like" characteristics, in the form of expressions favouring 
      synthesis of several neuromodulators/neurotransmitters, and as BDNF especially is 
      produced in neurons, it is of interest to confirm this. In the present study, 
      therefore, in situ hybridization for demonstration of BDNF mRNA was performed on 
      biopsies from Achilles tendons of patients with tendinosis and pain-free 
      non-tendinosis individuals. The results showed that the tenocytes of both groups 
      exhibited BDNF mRNA reactions. These observations indeed favour the idea that 
      BDNF is produced by tenocytes in the human Achilles tendon, why Achilles tendon 
      tissue is a tissue in which BDNF can be locally produced. BDNF can have 
      modulatory functions for the tenocytes, including apoptosis-modifying effects via 
      actions on the p75 receptor and interactive effects with 
      neurotransmitters/neuromodulators produced in these cells. This possibility 
      should be further studied for Achilles tendon tissue.
FAU - Bagge, Johan
AU  - Bagge J
AD  - Department of Integrative Medical Biology, Section for Anatomy, Umea University, 
      Umea, Sweden. johan.bagge@anatomy.umu.se
FAU - Danielson, Patrik
AU  - Danielson P
FAU - Forsgren, Sture
AU  - Forsgren S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - Histol Histopathol
JT  - Histology and histopathology
JID - 8609357
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Achilles Tendon/*metabolism
MH  - Adult
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/analysis
EDAT- 2012/07/19 06:00
MHDA- 2012/11/14 06:00
CRDT- 2012/07/19 06:00
PHST- 2012/07/19 06:00 [entrez]
PHST- 2012/07/19 06:00 [pubmed]
PHST- 2012/11/14 06:00 [medline]
AID - 10.14670/HH-27.1239 [doi]
PST - ppublish
SO  - Histol Histopathol. 2012 Sep;27(9):1239-46. doi: 10.14670/HH-27.1239.