PMID- 22810062
OWN - NLM
STAT- MEDLINE
DCOM- 20130830
LR  - 20211021
IS  - 1751-7370 (Electronic)
IS  - 1751-7362 (Print)
IS  - 1751-7362 (Linking)
VI  - 7
IP  - 1
DP  - 2013 Jan
TI  - Link between microbial composition and carbon substrate-uptake preferences in a 
      PHA-storing community.
PG  - 1-12
LID - 10.1038/ismej.2012.74 [doi]
AB  - The microbial community of a fermented molasses-fed sequencing batch reactor 
      (SBR) operated under feast and famine conditions for production of 
      polyhydroxyalkanoates (PHAs) was identified and quantified through a 16 S rRNA 
      gene clone library and fluorescence in situ hybridization (FISH). The microbial 
      enrichment was found to be composed of PHA-storing populations (84% of the 
      microbial community), comprising members of the genera Azoarcus, Thauera and 
      Paracoccus. The dominant PHA-storing populations ensured the high functional 
      stability of the system (characterized by high PHA-storage efficiency, up to 60% 
      PHA content). The fermented molasses contained primarily acetate, propionate, 
      butyrate and valerate. The substrate preferences were determined by 
      microautoradiography-FISH and differences in the substrate-uptake capabilities 
      for the various probe-defined populations were found. The results showed that in 
      the presence of multiple substrates, microbial populations specialized in 
      different substrates were selected, thereby co-existing in the SBR by adapting to 
      different niches. Azoarcus and Thauera, primarily consumed acetate and butyrate, 
      respectively. Paracoccus consumed a broader range of substrates and had a higher 
      cell-specific substrate uptake. The relative species composition and their 
      substrate specialization were reflected in the substrate removal rates of 
      different volatile fatty acids in the SBR reactor.
FAU - Albuquerque, Maria G E
AU  - Albuquerque MG
AD  - REQUIMTE/CQFB, Departamento de Quimica, Faculdade de Ciencias e Tecnologia, 
      Universidade Nova de Lisboa, Lisboa, Portugal.
FAU - Carvalho, Gilda
AU  - Carvalho G
FAU - Kragelund, Caroline
AU  - Kragelund C
FAU - Silva, Ana F
AU  - Silva AF
FAU - Barreto Crespo, Maria T
AU  - Barreto Crespo MT
FAU - Reis, Maria A M
AU  - Reis MA
FAU - Nielsen, Per H
AU  - Nielsen PH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120719
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
RN  - 0 (Acetates)
RN  - 0 (Butyrates)
RN  - 0 (Polyhydroxyalkanoates)
RN  - 0 (Propionates)
SB  - IM
MH  - Acetates/metabolism
MH  - *Bioreactors
MH  - Butyrates/metabolism
MH  - Fermentation
MH  - In Situ Hybridization, Fluorescence
MH  - Molasses
MH  - Polyhydroxyalkanoates/*metabolism
MH  - Propionates/metabolism
MH  - Proteobacteria/*classification/genetics/isolation & purification/*metabolism
PMC - PMC3526170
EDAT- 2012/07/20 06:00
MHDA- 2013/08/31 06:00
PMCR- 2014/01/01
CRDT- 2012/07/20 06:00
PHST- 2012/07/20 06:00 [entrez]
PHST- 2012/07/20 06:00 [pubmed]
PHST- 2013/08/31 06:00 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - ismej201274 [pii]
AID - 10.1038/ismej.2012.74 [doi]
PST - ppublish
SO  - ISME J. 2013 Jan;7(1):1-12. doi: 10.1038/ismej.2012.74. Epub 2012 Jul 19.