PMID- 22810222
OWN - NLM
STAT- MEDLINE
DCOM- 20121119
LR  - 20211021
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 287
IP  - 36
DP  - 2012 Aug 31
TI  - Structures of the PelD cyclic diguanylate effector involved in pellicle formation 
      in Pseudomonas aeruginosa PAO1.
PG  - 30191-204
LID - 10.1074/jbc.M112.378273 [doi]
AB  - The second messenger bis-(3'-5')-cyclic dimeric guanosine monophosphate 
      (c-di-GMP) plays a vital role in the global regulation in bacteria. Here, we 
      describe structural and biochemical characterization of a novel c-di-GMP effector 
      PelD that is critical to the formation of pellicles by Pseudomonas aeruginosa. We 
      present high-resolution structures of a cytosolic fragment of PelD in apo form 
      and its complex with c-di-GMP. The structure contains a bi-domain architecture 
      composed of a GAF domain (commonly found in cyclic nucleotide receptors) and a 
      GGDEF domain (found in c-di-GMP synthesizing enzymes), with the latter binding to 
      one molecule of c-di-GMP. The GGDEF domain has a degenerate active site but a 
      conserved allosteric site (I-site), which we show binds c-di-GMP with a K(d) of 
      0.5 mum. We identified a series of residues that are crucial for c-di-GMP binding, 
      and confirmed the roles of these residues through biochemical characterization of 
      site-specific variants. The structures of PelD represent a novel class of 
      c-di-GMP effector and expand the knowledge of scaffolds that mediate c-di-GMP 
      recognition.
FAU - Li, Zhi
AU  - Li Z
AD  - Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, 
      IL 61801, USA.
FAU - Chen, Jui-Hui
AU  - Chen JH
FAU - Hao, Yue
AU  - Hao Y
FAU - Nair, Satish K
AU  - Nair SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120717
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Bacterial Proteins)
RN  - 0 (Carrier Proteins)
RN  - 61093-23-0 (bis(3',5')-cyclic diguanylic acid)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Bacterial Proteins/*chemistry/genetics/metabolism
MH  - Carrier Proteins/*chemistry/genetics/metabolism
MH  - Cell Wall/*chemistry/genetics/metabolism
MH  - Crystallography, X-Ray
MH  - Cyclic GMP/*analogs & derivatives/chemistry/genetics/metabolism
MH  - Protein Structure, Tertiary
MH  - Pseudomonas aeruginosa/*chemistry/genetics/metabolism
PMC - PMC3436273
EDAT- 2012/07/20 06:00
MHDA- 2012/12/10 06:00
PMCR- 2013/08/31
CRDT- 2012/07/20 06:00
PHST- 2012/07/20 06:00 [entrez]
PHST- 2012/07/20 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
PHST- 2013/08/31 00:00 [pmc-release]
AID - S0021-9258(20)63123-4 [pii]
AID - M112.378273 [pii]
AID - 10.1074/jbc.M112.378273 [doi]
PST - ppublish
SO  - J Biol Chem. 2012 Aug 31;287(36):30191-204. doi: 10.1074/jbc.M112.378273. Epub 
      2012 Jul 17.