PMID- 22812678
OWN - NLM
STAT- MEDLINE
DCOM- 20130115
LR  - 20220330
IS  - 1557-7465 (Electronic)
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 32
IP  - 9
DP  - 2012 Sep
TI  - Ethanol exposure suppresses bone marrow-derived dendritic cell inflammatory 
      responses independent of TLR4 expression.
PG  - 416-25
LID - 10.1089/jir.2012.0005 [doi]
AB  - Acute alcohol (ethanol) exposure is linked with increased susceptibility to 
      infection and increased mortality in trauma and burn patients. Dendritic cells 
      (DCs) are central mediators in innate and adaptive immune responses, and they 
      play a role in the presentation of pathogens to adaptive immune cells. We 
      investigated the effects of acute ethanol exposure on bone marrow-derived DC 
      (BM-DC) responses. Total bone marrow cells, obtained from 8 to 10 week old 
      C57BL/6 male mice, were cultured in the presence of granulocyte/monocyte-colony 
      stimulating factor and interleukin (IL)-4 for 7 days. BM-DCs were harvested and 
      treated with increasing doses of ethanol (50, 100, and 250 mM) at the time of, or 
      3 h before, lipopolysaccharide (LPS). After LPS, supernatants were collected for 
      cytokine measurement, and cells were harvested for flow cytometry. Concurrent 
      acute ethanol exposure and LPS treatment resulted in a dose-dependent suppression 
      of IL-6, IL-12p40, IL-23, and IL-10. In addition, ethanol exposure before LPS 
      dysregulated the IL-12p40/IL-23 balance and more profoundly suppressed IL-6 and 
      IL-10 secretion by BM-DCs, as compared with cells concurrently treated with 
      ethanol and LPS. Ethanol treatment did not affect either toll-like receptor 
      (TLR)4 or TLR2 expression. In summary, our study demonstrates that acute ethanol 
      exposure suppresses BM-DC LPS-induced responses, irrespective of affecting TLR4 
      or TLR2 expression.
FAU - Rendon, Juan L
AU  - Rendon JL
AD  - Health Sciences Division, Alcohol Research Program, Loyola University Chicago, 
      Maywood, Illinois 60153, USA.
FAU - Janda, Brian A
AU  - Janda BA
FAU - Bianco, Monica E
AU  - Bianco ME
FAU - Choudhry, Mashkoor A
AU  - Choudhry MA
LA  - eng
GR  - R01AA015731/AA/NIAAA NIH HHS/United States
GR  - F30AA020167/AA/NIAAA NIH HHS/United States
GR  - T32AA013527/AA/NIAAA NIH HHS/United States
GR  - R01AA015731-04S1/AA/NIAAA NIH HHS/United States
GR  - F30 AA020167/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120719
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Central Nervous System Depressants)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 207137-56-2 (Interleukin-4)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/immunology/*metabolism/pathology
MH  - Central Nervous System Depressants/*pharmacology
MH  - Dendritic Cells/immunology/*metabolism/pathology
MH  - Ethanol/*pharmacology
MH  - Gene Expression Regulation/*drug effects/immunology
MH  - Inflammation/immunology/metabolism/pathology
MH  - Interleukin-4/immunology/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Mice
MH  - Toll-Like Receptor 2/biosynthesis/immunology
MH  - Toll-Like Receptor 4/*biosynthesis/immunology
PMC - PMC3438840
EDAT- 2012/07/21 06:00
MHDA- 2013/01/16 06:00
PMCR- 2013/09/01
CRDT- 2012/07/21 06:00
PHST- 2012/07/21 06:00 [entrez]
PHST- 2012/07/21 06:00 [pubmed]
PHST- 2013/01/16 06:00 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - 10.1089/jir.2012.0005 [pii]
AID - 10.1089/jir.2012.0005 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2012 Sep;32(9):416-25. doi: 10.1089/jir.2012.0005. 
      Epub 2012 Jul 19.