PMID- 22814531
OWN - NLM
STAT- MEDLINE
DCOM- 20121207
LR  - 20120924
IS  - 1462-0332 (Electronic)
IS  - 1462-0324 (Linking)
VI  - 51
IP  - 10
DP  - 2012 Oct
TI  - Myeloperoxidase and oxidative stress in rheumatoid arthritis.
PG  - 1796-803
AB  - OBJECTIVE: To determine whether MPO contributes to oxidative stress and disease 
      activity in RA and whether it produces hypochlorous acid in SF. METHODS: Plasma 
      and where possible SF were collected from 77 RA patients while 120 healthy 
      controls supplied plasma only. MPO and protein carbonyls were measured by ELISAs. 
      3-Chlorotyrosine in proteins and allantoin in plasma were measured by mass 
      spectrometry. RESULTS: Plasma MPO concentrations were significantly higher in 
      patients with RA compared with healthy controls [10.8 ng/ml, inter-quartile range 
      (IQR): 7.2-14.2; P<0.05], but there was no significant difference in plasma MPO 
      protein concentrations between RA patients with high disease activity (HDA; 
      DAS-28 >3.2) and those with low disease activity (LDA; DAS-28 </= 3.2) (HDA 27.9 
      ng/ml, 20.2-34.1 vs LDA 22.1 ng/ml, 16.9-34.9; P>0.05). There was a significant 
      relationship between plasma MPO and DAS-28 (r=0.35; P=0.005). Plasma protein 
      carbonyls and allantoin were significantly higher in patients with RA compared 
      with the healthy controls. MPO protein was significantly higher in SF compared 
      with plasma (median 624.0 ng/ml, IQR 258.4-2433.0 vs 30.2 ng/ml, IQR 25.1-50.9; 
      P<0.0001). The MPO present in SF was mostly active. 3-Chlorotyrosine, a specific 
      biomarker of hypochlorous acid, was present in proteins from SF and related to 
      the concentration of MPO (r=0.69; P=0.001). Protein carbonyls in SF were 
      associated with MPO protein concentration (r=0.40; P=0.019) and 3-chlorotyrosine 
      (r=0.66; P=0.003). CONCLUSION: MPO is elevated in patients with RA and promotes 
      oxidative stress through the production of hypochlorous acid.
FAU - Stamp, Lisa K
AU  - Stamp LK
AD  - Department of Medicine, University of Otago, Christchurch, Christchurch 8140, New 
      Zealand. lisa.stamp@cdhb.govt.nz
FAU - Khalilova, Irada
AU  - Khalilova I
FAU - Tarr, Joanna M
AU  - Tarr JM
FAU - Senthilmohan, Revathy
AU  - Senthilmohan R
FAU - Turner, Rufus
AU  - Turner R
FAU - Haigh, Richard C
AU  - Haigh RC
FAU - Winyard, Paul G
AU  - Winyard PG
FAU - Kettle, Anthony J
AU  - Kettle AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120719
PL  - England
TA  - Rheumatology (Oxford)
JT  - Rheumatology (Oxford, England)
JID - 100883501
RN  - 0 (Reactive Oxygen Species)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Arthritis, Rheumatoid/blood/*metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Oxidative Stress/*physiology
MH  - Peroxidase/blood/*metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Synovial Fluid/*metabolism
EDAT- 2012/07/21 06:00
MHDA- 2012/12/12 06:00
CRDT- 2012/07/21 06:00
PHST- 2012/07/21 06:00 [entrez]
PHST- 2012/07/21 06:00 [pubmed]
PHST- 2012/12/12 06:00 [medline]
AID - kes193 [pii]
AID - 10.1093/rheumatology/kes193 [doi]
PST - ppublish
SO  - Rheumatology (Oxford). 2012 Oct;51(10):1796-803. doi: 
      10.1093/rheumatology/kes193. Epub 2012 Jul 19.