PMID- 22815262
OWN - NLM
STAT- MEDLINE
DCOM- 20130618
LR  - 20211021
IS  - 1526-968X (Electronic)
IS  - 1526-954X (Print)
IS  - 1526-954X (Linking)
VI  - 50
IP  - 12
DP  - 2012 Dec
TI  - Microarray analysis of Xenopus endoderm expressing Ptf1a.
PG  - 853-70
LID - 10.1002/dvg.22048 [doi]
AB  - Pancreas-specific transcription factor 1a (Ptf1a), a bHLH transcription factor, 
      has two temporally distinct functions during pancreas development; initially it 
      is required for early specification of the entire pancreas, while later it is 
      required for proper differentiation and maintenance of only acinar cells. The 
      importance of Ptf1a function was revealed by the fact that loss of Ptf1a leads to 
      pancreas agenesis in humans. While Ptf1a is one of the most important pancreatic 
      transcription factors, little is known about the differences between the 
      regulatory networks it controls during initial specification of the pancreas as 
      opposed to acinar cell development, and to date no comprehensive analysis of its 
      downstream targets has been published. In this article, we use Xenopus embryos to 
      identify putative downstream targets of Ptf1a. We isolated anterior endoderm 
      tissue overexpressing Ptf1a at two early stages, NF32 and NF36, and compared 
      their gene expression profiles using microarrays. Our results revealed that Ptf1a 
      regulates genes with a wide variety of functions, providing insight into the 
      complexity of the regulatory network required for pancreas specification.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Bilogan, Cassandra K
AU  - Bilogan CK
AD  - Bell Center for Regenerative Biology and Tissue Engineering, Marine Biological 
      Laboratory, Woods Hole, Massachusetts, USA.
FAU - Horb, Marko E
AU  - Horb ME
LA  - eng
GR  - R01 DK077197/DK/NIDDK NIH HHS/United States
GR  - DK077197/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120816
PL  - United States
TA  - Genesis
JT  - Genesis (New York, N.Y. : 2000)
JID - 100931242
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Ptf1a protein, Xenopus)
RN  - 0 (Transcription Factors)
RN  - 0 (Xenopus Proteins)
RN  - 0 (transcription factor PTF1)
SB  - IM
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/genetics/*metabolism
MH  - Endoderm/cytology/*metabolism
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Developmental
MH  - Oligonucleotide Array Sequence Analysis
MH  - Pancreas/embryology/metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - Xenopus Proteins/genetics/*metabolism
MH  - Xenopus laevis
PMC - PMC3498591
MID - NIHMS396651
EDAT- 2012/07/21 06:00
MHDA- 2013/06/19 06:00
PMCR- 2013/12/01
CRDT- 2012/07/21 06:00
PHST- 2011/08/01 00:00 [received]
PHST- 2012/07/03 00:00 [revised]
PHST- 2012/07/09 00:00 [accepted]
PHST- 2012/07/21 06:00 [entrez]
PHST- 2012/07/21 06:00 [pubmed]
PHST- 2013/06/19 06:00 [medline]
PHST- 2013/12/01 00:00 [pmc-release]
AID - 10.1002/dvg.22048 [doi]
PST - ppublish
SO  - Genesis. 2012 Dec;50(12):853-70. doi: 10.1002/dvg.22048. Epub 2012 Aug 16.