PMID- 22815285
OWN - NLM
STAT- MEDLINE
DCOM- 20121019
LR  - 20211021
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 189
IP  - 4
DP  - 2012 Aug 15
TI  - A critical role for Rictor in T lymphopoiesis.
PG  - 1850-7
LID - 10.4049/jimmunol.1201057 [doi]
AB  - Apart from a critical role for Notch and pre-TCR, the signaling pathway required 
      for T lymphopoiesis is largely unknown. Given the potential link between Notch 
      and mammalian target of rapamycin (mTOR) signaling in cancer cells, we used mice 
      with conditional deletion of either Raptor or Rictor genes to determine potential 
      contribution of the mTOR complex I and II in T lymphopoiesis. Our data 
      demonstrated that targeted mutation of Rictor in the thymocytes drastically 
      reduced the thymic cellularity, primarily by reducing proliferation of the 
      immature thymocytes. Rictor deficiency caused a partial block of thymocyte 
      development at the double-negative 3 stage. The effect of Rictor deficiency is 
      selective for the T cell lineage, as the development of B cells, erythrocytes, 
      and myeloid cells is largely unaffected. Analysis of bone marrow chimera 
      generated from a mixture of wild-type and Rictor-deficient hematopoietic stem 
      cells demonstrated that the function of Rictor is cell intrinsic. These data 
      revealed a critical function of mTOR complex 2 in T lymphopoiesis.
FAU - Tang, Fei
AU  - Tang F
AD  - Division of Immunotherapy, Department of Surgery, University of Michigan Medical 
      School and Comprehensive Cancer Center, Ann Arbor, MI 48109, USA.
FAU - Wu, Qi
AU  - Wu Q
FAU - Ikenoue, Tsuneo
AU  - Ikenoue T
FAU - Guan, Kun-Liang
AU  - Guan KL
FAU - Liu, Yang
AU  - Liu Y
FAU - Zheng, Pan
AU  - Zheng P
LA  - eng
GR  - R01 CA058033/CA/NCI NIH HHS/United States
GR  - R01 AI064350/AI/NIAID NIH HHS/United States
GR  - R01 AG036690/AG/NIA NIH HHS/United States
GR  - R01AG036690/AG/NIA NIH HHS/United States
GR  - AI64350/AI/NIAID NIH HHS/United States
GR  - RC1 GM091648/GM/NIGMS NIH HHS/United States
GR  - CA58033/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120718
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Rapamycin-Insensitive Companion of mTOR Protein)
RN  - 0 (Regulatory-Associated Protein of mTOR)
RN  - 0 (Rptor protein, mouse)
RN  - 0 (rictor protein, mouse)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Carrier Proteins/*immunology/metabolism
MH  - Cell Differentiation/genetics/*immunology
MH  - Flow Cytometry
MH  - Lymphopoiesis/genetics/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Precursor Cells, T-Lymphoid/cytology/*immunology
MH  - Rapamycin-Insensitive Companion of mTOR Protein
MH  - Real-Time Polymerase Chain Reaction
MH  - Regulatory-Associated Protein of mTOR
MH  - T-Lymphocytes/cytology/*immunology
PMC - PMC3412163
MID - NIHMS388117
EDAT- 2012/07/21 06:00
MHDA- 2012/10/20 06:00
PMCR- 2013/08/15
CRDT- 2012/07/21 06:00
PHST- 2012/07/21 06:00 [entrez]
PHST- 2012/07/21 06:00 [pubmed]
PHST- 2012/10/20 06:00 [medline]
PHST- 2013/08/15 00:00 [pmc-release]
AID - jimmunol.1201057 [pii]
AID - 10.4049/jimmunol.1201057 [doi]
PST - ppublish
SO  - J Immunol. 2012 Aug 15;189(4):1850-7. doi: 10.4049/jimmunol.1201057. Epub 2012 
      Jul 18.