PMID- 22815499
OWN - NLM
STAT- MEDLINE
DCOM- 20121024
LR  - 20211021
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 32
IP  - 29
DP  - 2012 Jul 18
TI  - Platelet-derived growth factor-BB restores human immunodeficiency virus 
      Tat-cocaine-mediated impairment of neurogenesis: role of TRPC1 channels.
PG  - 9835-47
LID - 10.1523/JNEUROSCI.0638-12.2012 [doi]
AB  - Platelet-derived growth factor-BB (PDGF-BB) has been reported to provide tropic 
      support for neurons in the CNS. However, whether PDGF-BB regulates neurogenesis, 
      especially in the context of HIV-associated neurological disorder and drug abuse, 
      remains essentially unknown. In this study, we demonstrate that pretreatment of 
      rat hippocampal neuronal progenitor cells (NPCs) with PDGF-BB restored 
      proliferation that had been impaired by HIV Tat-cocaine via the cognate 
      receptors. We identify the essential role of transient receptor potential 
      canonical (TRPC) channels in PDGF-BB-mediated proliferation. Parallel but 
      distinct ERK/CREB, phosphatidylinositol 3-kinase/Akt signaling pathways with 
      downstream activation of mammalian target of rapamycin (mTOR)/eukaryotic 
      translation initiation factor 4E-binding protein (4E-BP)-p70S6K and nuclear 
      factor-kappaB were critical for proliferation. Blocking TRPC1 channel suppressed 
      PDGF-mediated proliferation as well as PDGF-BB-induced ERK/CREB and 
      mTOR/4E-BP-p70S6K activation, thereby underscoring its role in this process. In 
      vivo relevance of these findings was further corroborated in Tat transgenic mice 
      wherein hippocampal injection of recombinant AAV2-PDGF-B restored impaired NPC 
      proliferation that was induced by Tat-cocaine. Together, these data underpin the 
      role of TRPC1 channel as a novel target that regulates cell proliferation 
      mediated by PDGF-BB with implications for therapeutic intervention for reversal 
      of impaired neurogenesis inflicted by Tat and cocaine.
FAU - Yao, Honghong
AU  - Yao H
AD  - Department of Pharmacology and Experimental Neuroscience, University of Nebraska 
      Medical Center, Omaha, Nebraska 68198, USA. hyao@unmc.edu
FAU - Duan, Ming
AU  - Duan M
FAU - Yang, Lu
AU  - Yang L
FAU - Buch, Shilpa
AU  - Buch S
LA  - eng
GR  - R01 DA024442/DA/NIDA NIH HHS/United States
GR  - R21 DA033614/DA/NIDA NIH HHS/United States
GR  - P30 MH062261/MH/NIMH NIH HHS/United States
GR  - DA 033150/DA/NIDA NIH HHS/United States
GR  - DA024442/DA/NIDA NIH HHS/United States
GR  - DA030285/DA/NIDA NIH HHS/United States
GR  - R21 DA030285/DA/NIDA NIH HHS/United States
GR  - R01 DA033150/DA/NIDA NIH HHS/United States
GR  - DA 033614/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Dopamine Uptake Inhibitors)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 0 (TRPC Cation Channels)
RN  - 0 (tat Gene Products, Human Immunodeficiency Virus)
RN  - 0 (transient receptor potential cation channel, subfamily C, member 1)
RN  - 1B56C968OA (Becaplermin)
RN  - I5Y540LHVR (Cocaine)
SB  - IM
MH  - Animals
MH  - Becaplermin
MH  - Cell Proliferation/drug effects
MH  - Cocaine/*pharmacology
MH  - Dopamine Uptake Inhibitors/*pharmacology
MH  - Hippocampus/cytology/drug effects/metabolism
MH  - Neural Stem Cells/cytology/*drug effects/metabolism
MH  - Neurogenesis/*drug effects/physiology
MH  - Neurons/cytology/drug effects/metabolism
MH  - Proto-Oncogene Proteins c-sis/*pharmacology
MH  - RNA Interference
MH  - Rats
MH  - TRPC Cation Channels/genetics/*metabolism
MH  - tat Gene Products, Human Immunodeficiency Virus/*pharmacology
PMC - PMC3438664
MID - NIHMS395457
EDAT- 2012/07/21 06:00
MHDA- 2012/10/25 06:00
PMCR- 2013/01/18
CRDT- 2012/07/21 06:00
PHST- 2012/07/21 06:00 [entrez]
PHST- 2012/07/21 06:00 [pubmed]
PHST- 2012/10/25 06:00 [medline]
PHST- 2013/01/18 00:00 [pmc-release]
AID - 32/29/9835 [pii]
AID - 3787418 [pii]
AID - 10.1523/JNEUROSCI.0638-12.2012 [doi]
PST - ppublish
SO  - J Neurosci. 2012 Jul 18;32(29):9835-47. doi: 10.1523/JNEUROSCI.0638-12.2012.