PMID- 22815834
OWN - NLM
STAT- MEDLINE
DCOM- 20130321
LR  - 20220316
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 7
DP  - 2012
TI  - Effects of hypoxia exposure on hepatic cytochrome P450 1A (CYP1A) expression in 
      Atlantic croaker: molecular mechanisms of CYP1A down-regulation.
PG  - e40825
LID - 10.1371/journal.pone.0040825 [doi]
LID - e40825
AB  - Hypoxia-inducible factor-alpha (HIF-alpha) and cytochrome P450 1A (CYP1A) are biomarkers 
      of environmental exposure to hypoxia and organic xenobiotic chemicals that act 
      through the aryl hydrocarbon receptor, respectively. Many aquatic environments 
      heavily contaminated with organic chemicals, such as harbors, are also hypoxic. 
      Recently, we and other scientists reported HIF-alpha genes are upregulated by hypoxia 
      exposure in aquatic organisms, but the molecular mechanisms of hypoxia regulation 
      of CYP1A expression have not been investigated in teleost fishes. As a first step 
      in understanding the molecular mechanisms of hypoxia modulation of CYP1A 
      expression in fish, we characterized CYP1A cDNA from croaker liver. Hypoxia 
      exposure (dissolved oxygen, DO: 1.7 mg/L for 2 to 4 weeks) caused significant 
      decreases in hepatic CYP1A mRNA and protein levels compared to CYP1A levels in 
      fish held in normoxic conditions. In vivo studies showed that the nitric oxide 
      (NO)-donor, S-nitroso-N-acetyl-DL-penicillamine, significantly decreased CYP1A 
      expression in croaker livers, whereas the competitive inhibitor of NO synthase 
      (NOS), N(omega)-nitro-L-arginine methyl ester, restored CYP1A mRNA and protein levels 
      in hypoxia-exposed (1.7 mg DO/L for 4 weeks) fish. In vivo hypoxia exposure also 
      markedly increased interleukin-1beta (IL-1beta, a cytokine), HIF-2alpha mRNA and 
      endothelial NOS (eNOS) protein levels in croaker livers. Pharmacological 
      treatment with vitamin E, an antioxidant, lowered the IL-1beta, HIF-2alpha mRNA and eNOS 
      protein levels in hypoxia-exposed fish and completely reversed the 
      down-regulation of hepatic CYP1A mRNA and protein levels in response to hypoxia 
      exposure. These results suggest that hypoxia-induced down-regulation of CYP1A is 
      due to alterations of NO and oxidant status, and cellular IL-1beta and HIF-alpha levels. 
      Moreover, the present study provides the first evidence of a role for 
      antioxidants in hepatic eNOS and IL-1beta regulation in aquatic vertebrates during 
      hypoxic stress.
FAU - Rahman, Md Saydur
AU  - Rahman MS
AD  - Marine Science Institute, University of Texas at Austin, Port Aransas, Texas, 
      United States of America. rahman@mail.utexas.edu
FAU - Thomas, Peter
AU  - Thomas P
LA  - eng
SI  - GENBANK/JQ622219
SI  - GENBANK/JQ622220
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120716
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antioxidants)
RN  - 0 (RNA, Messenger)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Atlantic Ocean
MH  - Blotting, Western
MH  - Cytochrome P-450 Enzyme System/*genetics/metabolism
MH  - Down-Regulation/drug effects/*genetics
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Enzymologic
MH  - Hypoxia/*enzymology/*genetics
MH  - Immunohistochemistry
MH  - Liver/drug effects/*enzymology
MH  - Models, Biological
MH  - Molecular Sequence Data
MH  - Nitric Oxide/pharmacology
MH  - Nitric Oxide Synthase Type III/metabolism
MH  - Organ Specificity/drug effects/genetics
MH  - Perciformes/*genetics
MH  - Protein Transport/drug effects/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
MH  - Tissue Distribution/drug effects/genetics
PMC - PMC3397942
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/07/21 06:00
MHDA- 2013/03/22 06:00
PMCR- 2012/07/16
CRDT- 2012/07/21 06:00
PHST- 2012/04/17 00:00 [received]
PHST- 2012/06/13 00:00 [accepted]
PHST- 2012/07/21 06:00 [entrez]
PHST- 2012/07/21 06:00 [pubmed]
PHST- 2013/03/22 06:00 [medline]
PHST- 2012/07/16 00:00 [pmc-release]
AID - PONE-D-12-11055 [pii]
AID - 10.1371/journal.pone.0040825 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(7):e40825. doi: 10.1371/journal.pone.0040825. Epub 2012 Jul 16.