PMID- 22817758
OWN - NLM
STAT- MEDLINE
DCOM- 20130514
LR  - 20240317
IS  - 1471-2180 (Electronic)
IS  - 1471-2180 (Linking)
VI  - 12
DP  - 2012 Jul 20
TI  - Comparison of oral microbiota in tumor and non-tumor tissues of patients with 
      oral squamous cell carcinoma.
PG  - 144
LID - 10.1186/1471-2180-12-144 [doi]
AB  - BACKGROUND: Bacterial infections have been linked to malignancies due to their 
      ability to induce chronic inflammation. We investigated the association of oral 
      bacteria in oral squamous cell carcinoma (OSCC/tumor) tissues and compared with 
      adjacent non-tumor mucosa sampled 5 cm distant from the same patient (n = 10). By 
      using culture-independent 16S rRNA approaches, denaturing gradient gel 
      electrophoresis (DGGE) and cloning and sequencing, we assessed the total 
      bacterial diversity in these clinical samples. RESULTS: DGGE fingerprints showed 
      variations in the band intensity profiles within non-tumor and tumor tissues of 
      the same patient and among the two groups. The clonal analysis indicated that 
      from a total of 1200 sequences characterized, 80 bacterial species/phylotypes 
      were detected representing six phyla, Firmicutes, Bacteroidetes, Proteobacteria, 
      Fusobacteria, Actinobacteria and uncultivated TM7 in non-tumor and tumor 
      libraries. In combined library, 12 classes, 16 order, 26 families and 40 genera 
      were observed. Bacterial species, Streptococcus sp. oral taxon 058, 
      Peptostreptococcus stomatis, Streptococcus salivarius, Streptococcus gordonii, 
      Gemella haemolysans, Gemella morbillorum, Johnsonella ignava and Streptococcus 
      parasanguinis I were highly associated with tumor site where as Granulicatella 
      adiacens was prevalent at non-tumor site. Streptococcus intermedius was present 
      in 70% of both non-tumor and tumor sites. CONCLUSIONS: The underlying changes in 
      the bacterial diversity in the oral mucosal tissues from non-tumor and tumor 
      sites of OSCC subjects indicated a shift in bacterial colonization. These most 
      prevalent or unique bacterial species/phylotypes present in tumor tissues may be 
      associated with OSCC and needs to be further investigated with a larger sample 
      size.
FAU - Pushalkar, Smruti
AU  - Pushalkar S
AD  - Department of Basic Sciences and Craniofacial Biology, New York University 
      College of Dentistry, 345 E, 24th Street, Room 921B, New York, NY 10010, USA.
FAU - Ji, Xiaojie
AU  - Ji X
FAU - Li, Yihong
AU  - Li Y
FAU - Estilo, Cherry
AU  - Estilo C
FAU - Yegnanarayana, Ramanathan
AU  - Yegnanarayana R
FAU - Singh, Bhuvanesh
AU  - Singh B
FAU - Li, Xin
AU  - Li X
FAU - Saxena, Deepak
AU  - Saxena D
LA  - eng
GR  - DE019178/DE/NIDCR NIH HHS/United States
GR  - DE020891/DE/NIDCR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120720
PL  - England
TA  - BMC Microbiol
JT  - BMC microbiology
JID - 100966981
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacteria/*classification/isolation & purification
MH  - *Biodiversity
MH  - Carcinoma, Squamous Cell/*microbiology/*pathology
MH  - Cloning, Molecular
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Female
MH  - Humans
MH  - Male
MH  - Metagenome
MH  - Middle Aged
MH  - Mouth Mucosa/*microbiology
MH  - Mouth Neoplasms/*microbiology/*pathology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
PMC - PMC3507910
EDAT- 2012/07/24 06:00
MHDA- 2013/05/15 06:00
PMCR- 2012/07/20
CRDT- 2012/07/24 06:00
PHST- 2012/02/10 00:00 [received]
PHST- 2012/07/20 00:00 [accepted]
PHST- 2012/07/24 06:00 [entrez]
PHST- 2012/07/24 06:00 [pubmed]
PHST- 2013/05/15 06:00 [medline]
PHST- 2012/07/20 00:00 [pmc-release]
AID - 1471-2180-12-144 [pii]
AID - 10.1186/1471-2180-12-144 [doi]
PST - epublish
SO  - BMC Microbiol. 2012 Jul 20;12:144. doi: 10.1186/1471-2180-12-144.