PMID- 22819550 OWN - NLM STAT- MEDLINE DCOM- 20130524 LR - 20211203 IS - 1873-4847 (Electronic) IS - 0955-2863 (Linking) VI - 24 IP - 1 DP - 2013 Jan TI - Quercetin intake during lactation modulates the AMP-activated protein kinase pathway in the livers of adult male rat offspring programmed by maternal protein restriction. PG - 118-23 LID - S0955-2863(12)00091-5 [pii] LID - 10.1016/j.jnutbio.2012.03.007 [doi] AB - Quercetin, a naturally occurring flavonoid, has been reported to possess numerous biological activities including activation of adenosine-5'-monophosphate-activated protein kinase (AMPK). We investigated the effects of quercetin intake during lactation on the AMPK activation in the livers of adult offspring programmed by maternal protein restriction during gestation. Pregnant Wistar rats were fed control and low-protein diets during gestation. Following delivery, each dam received a control or 0.2% quercetin-containing control diet during lactation as follows: control on control (CC), control on restricted (LPC) and 0.2% quercetin-containing control on restricted (LPQ). At weaning (week 3), some of the pups from each dam were killed, and the remaining pups (CC, n=8; LPC, n=10; LPQ, n=13) continued to receive a standard laboratory diet and were killed at week 23. Blood chemistry and phosphorylation levels of AMPKalpha, acetyl-CoA carboxylase (ACC), endothelial nitric oxide synthase (eNOS) and mammalian target of rapamycin (mTOR) in the livers of male offspring were examined. At week 3, the level of phosphorylated AMPK protein in LPQ increased about 1.5- and 2.1-fold compared with LPC and CC, respectively, and the level in LPQ at week 23 increased about 1.9- and 2.9-fold, respectively. A significant increase in phosphorylated ACC and eNOS levels was found in LPQ. There was no significant difference among the three groups in the level of phosphorylated mTOR protein. In conclusion, quercetin intake during lactation up-regulates AMPK activation in the adult offspring of protein-restricted dams and modulates the AMPK pathway in the liver. CI - Copyright (c) 2013 Elsevier Inc. All rights reserved. FAU - Sato, Shin AU - Sato S AD - Department of Nutrition, Faculty of Health Sciences, Aomori University of Health and Welfare, Aomori 030-8505, Japan. s_sato3@auhw.ac.jp FAU - Mukai, Yuuka AU - Mukai Y FAU - Saito, Takeshi AU - Saito T LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120721 PL - United States TA - J Nutr Biochem JT - The Journal of nutritional biochemistry JID - 9010081 RN - 0 (Nitrates) RN - 0 (Nitrites) RN - 9IKM0I5T1E (Quercetin) RN - EC 1.14.13.39 (Nitric Oxide Synthase Type III) RN - EC 1.14.13.39 (Nos3 protein, rat) RN - EC 2.7.1.1 (mTOR protein, rat) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) RN - EC 2.7.11.31 (AMP-Activated Protein Kinases) RN - EC 6.4.1.2 (Acetyl-CoA Carboxylase) SB - IM MH - AMP-Activated Protein Kinases/*metabolism MH - Acetyl-CoA Carboxylase/metabolism MH - Age Factors MH - Animals MH - Body Weight/drug effects MH - Diet, Protein-Restricted/*adverse effects MH - Female MH - *Lactation MH - Liver/*drug effects/*metabolism MH - Male MH - Nitrates/urine MH - Nitric Oxide Synthase Type III/metabolism MH - Nitrites/urine MH - Organ Size/drug effects MH - Phosphorylation MH - Pregnancy MH - Prenatal Exposure Delayed Effects MH - Quercetin/*pharmacology MH - Rats MH - Rats, Wistar MH - TOR Serine-Threonine Kinases/metabolism EDAT- 2012/07/24 06:00 MHDA- 2013/05/28 06:00 CRDT- 2012/07/24 06:00 PHST- 2011/07/13 00:00 [received] PHST- 2012/02/10 00:00 [revised] PHST- 2012/03/07 00:00 [accepted] PHST- 2012/07/24 06:00 [entrez] PHST- 2012/07/24 06:00 [pubmed] PHST- 2013/05/28 06:00 [medline] AID - S0955-2863(12)00091-5 [pii] AID - 10.1016/j.jnutbio.2012.03.007 [doi] PST - ppublish SO - J Nutr Biochem. 2013 Jan;24(1):118-23. doi: 10.1016/j.jnutbio.2012.03.007. Epub 2012 Jul 21.