PMID- 22819609
OWN - NLM
STAT- MEDLINE
DCOM- 20121113
LR  - 20120813
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 903
DP  - 2012 Aug 15
TI  - Simultaneous determination of three flavonoid C-glycosides in mice biosamples by 
      HPLC-ESI-MS method after oral administration of Abrus mollis extract and its 
      application to biodistribution studies.
PG  - 68-74
LID - 10.1016/j.jchromb.2012.06.044 [doi]
AB  - A simple HPLC-ESI-MS method was developed for the determination of vicenin-2 (1), 
      isoschaftoside (2), and schaftoside (3) of Abrus mollis extract in mice plasma 
      and tissues (heart, liver, spleen, lungs, and kidneys). The separation was 
      achieved by HPLC on a Shim-Pack CLC-ODS column with a mobile phase composed of 
      0.1% formic acid (mobile phase A, 72%) and methanol-isopropanol (9:1) (mobile 
      phase B, 28%). The electrospray source of the MS was operated in the selective 
      ion monitoring (SIM) mode at m/z 593 ([M-H](-)) for 1, and 563 ([M-H](-)) for 2 and 
      3, respectively. The limit of detection (LOD) of 1-3 was in the range of 8.5-12.6 
      ng/mL for plasma, and 32.5-49.4 ng/g for tissue tested. The limit of 
      quantification (LOQ) was found to be 25 ng/mL for plasma, and 100 ng/g for 
      tissue. The calibration curves were linear in all matrices (r(2) > 0.994) in the 
      concentration range of 25-500 ng/mL in plasma or 100-1250 ng/g in tissue, 
      respectively. Intra-day and inter-day precision studies demonstrate that the 
      method is precise with coefficients of variation intra-day and inter-day below 
      5.9 and 7.4% for all the samples, respectively. The recoveries of three flavonoid 
      C-glycosides ranged from 95.3 to 106.4% for plasma, and 92.6 to 107.3% for 
      tissues. Following oral administration of A. mollis extract to mice at a dose of 
      72 mg/kg, the concentrations of 1-3 in plasma and tissues were quantifiable in 
      bio-samples collected up to 180 min. The method described is suitable for studies 
      on the distribution of three flavonoid C-glycosides of A. mollis extract in 
      plasma and different tissues of mice.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Wang, Hao
AU  - Wang H
AD  - State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 
      Tongjiaxiang, Nanjing 210009, China. btwanghao@yahoo.com.cn
FAU - Jiang, Zhenzhou
AU  - Jiang Z
FAU - Du, Huibing
AU  - Du H
FAU - Liang, Chunyi
AU  - Liang C
FAU - Wang, Yuanchao
AU  - Wang Y
FAU - Zhang, Mohan
AU  - Zhang M
FAU - Zhang, Luyong
AU  - Zhang L
FAU - Ye, Wencai
AU  - Ye W
FAU - Li, Ping
AU  - Li P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120709
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Flavonoids)
RN  - 0 (Glycosides)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Abrus/*chemistry
MH  - Administration, Oral
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Flavonoids/analysis/*blood/chemistry/pharmacokinetics
MH  - Glycosides/analysis/*blood/chemistry/pharmacokinetics
MH  - Limit of Detection
MH  - Male
MH  - Mice
MH  - Plant Extracts/administration & dosage/analysis/blood/pharmacokinetics
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Tissue Distribution
EDAT- 2012/07/24 06:00
MHDA- 2012/11/14 06:00
CRDT- 2012/07/24 06:00
PHST- 2011/12/30 00:00 [received]
PHST- 2012/04/09 00:00 [revised]
PHST- 2012/06/28 00:00 [accepted]
PHST- 2012/07/24 06:00 [entrez]
PHST- 2012/07/24 06:00 [pubmed]
PHST- 2012/11/14 06:00 [medline]
AID - S1570-0232(12)00398-4 [pii]
AID - 10.1016/j.jchromb.2012.06.044 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Aug 15;903:68-74. doi: 
      10.1016/j.jchromb.2012.06.044. Epub 2012 Jul 9.