PMID- 22819726
OWN - NLM
STAT- MEDLINE
DCOM- 20130129
LR  - 20191210
IS  - 1873-4138 (Electronic)
IS  - 0309-1740 (Linking)
VI  - 92
IP  - 4
DP  - 2012 Dec
TI  - Development of QuEChERS-based extraction and liquid chromatography-tandem mass 
      spectrometry method for quantifying flumethasone residues in beef muscle.
PG  - 749-53
LID - 10.1016/j.meatsci.2012.06.033 [doi]
AB  - A rapid, specific, and sensitive method based on liquid 
      chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) in 
      the positive ion mode using multiple reaction monitoring (MRM) was developed and 
      validated to quantify flumethasone residues in beef muscle. Methods were compared 
      between the original as well as the EN quick, easy, cheap, effective, rugged, and 
      safe (QuEChERS)-based extraction. Good linearity was achieved at concentration 
      levels of 5-30 mug/kg. Estimated recovery rates at spiking levels of 5 and 10 
      mug/kg ranged from 72.1 to 84.6%, with relative standard deviations (RSDs)<7%. The 
      results of the inter-day study, which was performed by fortifying beef muscle 
      samples (n=18) on 3 separate days, showed an accuracy of 93.4-94.4%. The 
      precision (expressed as relative standard deviation values) for the inter-day 
      variation at two levels of fortification (10 and 20 mug/kg) was 1.9-5.2%. The 
      limit of detection (LOD) and limit of quantitation (LOQ) were 1.7 and 5 mug/kg, at 
      signal-to-noise ratios (S/Ns) of 3 and 10, respectively. The method was 
      successfully applied to analyze real samples obtained from large markets 
      throughout the Korean Peninsula. The method proved to be sensitive and reliable 
      and, thus, rendered an appropriate means for residue analysis studies.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Park, Ki Hun
AU  - Park KH
AD  - Natural Products Chemistry Laboratory, Biotechnology Research Institute, Chonnam 
      National University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
FAU - Choi, Jeong-Heui
AU  - Choi JH
FAU - Abd El-Aty, A M
AU  - Abd El-Aty AM
FAU - Cho, Soon-Kil
AU  - Cho SK
FAU - Park, Jong-Hyouk
AU  - Park JH
FAU - Kwon, Ki Sung
AU  - Kwon KS
FAU - Park, Hee Ra
AU  - Park HR
FAU - Kim, Hyung Soo
AU  - Kim HS
FAU - Shin, Ho-Chul
AU  - Shin HC
FAU - Kim, Mi Ra
AU  - Kim MR
FAU - Shim, Jae-Han
AU  - Shim JH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20120629
PL  - England
TA  - Meat Sci
JT  - Meat science
JID - 101160862
RN  - 0 (Glucocorticoids)
RN  - 0 (Tissue Extracts)
RN  - LR3CD8SX89 (Flumethasone)
SB  - IM
MH  - Animals
MH  - Calibration
MH  - Cattle
MH  - Chromatography, High Pressure Liquid
MH  - Cost Savings
MH  - Drug Residues/*analysis/isolation & purification
MH  - Flumethasone/*analysis/isolation & purification
MH  - *Food Contamination
MH  - Food Inspection/economics/*methods
MH  - Glucocorticoids/*analysis/isolation & purification
MH  - Limit of Detection
MH  - Meat/*analysis/economics
MH  - Muscle, Skeletal/*chemistry
MH  - Reproducibility of Results
MH  - Republic of Korea
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
MH  - Time Factors
MH  - Tissue Extracts/chemistry/isolation & purification
EDAT- 2012/07/24 06:00
MHDA- 2013/01/30 06:00
CRDT- 2012/07/24 06:00
PHST- 2012/02/01 00:00 [received]
PHST- 2012/06/19 00:00 [revised]
PHST- 2012/06/20 00:00 [accepted]
PHST- 2012/07/24 06:00 [entrez]
PHST- 2012/07/24 06:00 [pubmed]
PHST- 2013/01/30 06:00 [medline]
AID - S0309-1740(12)00245-8 [pii]
AID - 10.1016/j.meatsci.2012.06.033 [doi]
PST - ppublish
SO  - Meat Sci. 2012 Dec;92(4):749-53. doi: 10.1016/j.meatsci.2012.06.033. Epub 2012 
      Jun 29.