PMID- 22819798
OWN - NLM
STAT- MEDLINE
DCOM- 20130116
LR  - 20231113
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Print)
IS  - 0882-4010 (Linking)
VI  - 53
IP  - 3-4
DP  - 2012 Sep
TI  - Weak binder for MHC molecule is a potent Mycobacterium tuberculosis-specific CTL 
      epitope in the context of HLA-A24 allele.
PG  - 162-7
LID - 10.1016/j.micpath.2012.07.002 [doi]
AB  - Tuberculosis causes serious health problem for the world population. Antigenic 
      peptides selected by pathogen-specific cytotoxic T lymphocytes (CTLs) are 
      presented by major histocompatibility complex (MHC; or human leukocyte antigen 
      [HLA] in humans) molecules, and HLA-A restricted responses may be of interest for 
      vaccine development and the understanding of cellular immunity. A series of 
      peptides derived from the 10-KDa culture filtrate protein (CFP10) and the 6 kDa 
      early secretory antigenic target (ESAT-6) in the Mycobacterium tuberculosis (Mtb) 
      have been screened and a CTL epitope restricted by the human leukocyte antigen 
      HLA-A24, a common HLA allele in Asian people, has been identified. In this study, 
      we studied a panel of CFP10 and ESAT-6-derived peptides to identify those with 
      binding motifs for HLA-A24 molecules. The antigenicity of candidate peptides was 
      assessed with in vitro refolding tests and an enzyme-linked immunospot (ELISPOT) 
      assay, and by tetramer staining to determine the capacity to stimulate CTLs from 
      peripheral blood mononuclear cells (PBMCs) of HLA-A24-positive TB Patients. We 
      report that one novel candidate peptide at positions 5-14 of ESAT-6 of Mtb could 
      induce peptide-specific CTLs from PBMCs of HLA-A24-positive patients, but not 
      from HLA-A24-negative patients and HLA-A24-positive healthy controls. Identified 
      epitope is a weak binder for HLA-A24 molecule in a mini MHC refolding assay. 
      Since the peptide is presented by a common HLA class I molecule, it may be useful 
      for immunotherapy against Mtb infection and vaccine development in the large 
      population of Mtb-infected patients.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Wang, Yan
AU  - Wang Y
AD  - Department of Microbiology, Nanjing Center for Disease Prevention and Control, 
      Zizhulin 2, Nanjing 210003, China.
FAU - Sun, Meiyi
AU  - Sun M
FAU - He, Min
AU  - He M
FAU - Cui, Honglian
AU  - Cui H
FAU - Zhang, Junxian
AU  - Zhang J
FAU - Shi, Limin
AU  - Shi L
FAU - Wang, Wei
AU  - Wang W
FAU - Xu, Wenjiong
AU  - Xu W
FAU - Gao, Bin
AU  - Gao B
FAU - Ding, Jie
AU  - Ding J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120720
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Cytokines)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-A24 Antigen)
SB  - IM
EIN - Microb Pathog. 2013 Mar;56:60
MH  - Adolescent
MH  - Adult
MH  - Amino Acid Sequence
MH  - Cells, Cultured
MH  - Cytokines/immunology
MH  - Epitopes, T-Lymphocyte/genetics/*immunology
MH  - Female
MH  - Genes, MHC Class I
MH  - HLA-A24 Antigen/genetics/*immunology
MH  - Humans
MH  - Leukocytes, Mononuclear/immunology
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Mycobacterium tuberculosis/genetics/*immunology/isolation & purification
MH  - Species Specificity
MH  - Tuberculosis/genetics/*immunology/microbiology
MH  - Young Adult
PMC - PMC7127545
EDAT- 2012/07/24 06:00
MHDA- 2013/01/17 06:00
PMCR- 2012/07/20
CRDT- 2012/07/24 06:00
PHST- 2012/04/24 00:00 [received]
PHST- 2012/07/01 00:00 [revised]
PHST- 2012/07/03 00:00 [accepted]
PHST- 2012/07/24 06:00 [entrez]
PHST- 2012/07/24 06:00 [pubmed]
PHST- 2013/01/17 06:00 [medline]
PHST- 2012/07/20 00:00 [pmc-release]
AID - S0882-4010(12)00134-9 [pii]
AID - 10.1016/j.micpath.2012.07.002 [doi]
PST - ppublish
SO  - Microb Pathog. 2012 Sep;53(3-4):162-7. doi: 10.1016/j.micpath.2012.07.002. Epub 
      2012 Jul 20.