PMID- 22819890
OWN - NLM
STAT- MEDLINE
DCOM- 20121226
LR  - 20221207
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 433
DP  - 2012 Sep 1
TI  - Diversity of microbial communities in an attached-growth system using Bauxsol 
      pellets for wastewater treatment.
PG  - 383-9
LID - 10.1016/j.scitotenv.2012.06.079 [doi]
AB  - Columns of Bauxsol pellets were used in a field experiment as biomass support 
      particle for wastewater microbial communities. The attached microbial community 
      structure was analysed using denaturing gradient gel electrophoresis (DGGE), 
      targeting the 16S rDNA gene's V3 region. DGGE profiles showed that the type and 
      composition of support particles used (i.e. Bauxsol pellets or gravel) had a 
      significant impact on the attached bacterial communities (64% dissimilarity). In 
      addition, ecological indices revealed a more heterogeneous bacterial community 
      structure on the Bauxsol pellets. TOC/TN ratios post-experiment (6.5-9.3) 
      suggested a good level of biological activity (i.e. active biofilm) in the 
      Bauxsol columns. Moreover, Bauxsol pellets were mostly made of inorganic 
      carbon, suggesting insoluble carbonate biomineralisation. Polymerase chain 
      reaction (PCR) amplification of specific marker genes (i.e. bacterial and 
      archaeal amoA genes, nosZ gene, and hzo gene) were used to identify the presence 
      of attached bacterial communities associated with nitrogen transformation. The 
      results along with geochemical data (i.e. up to 50% nitrogen removal) revealed 
      co-existence of ammonia-oxidising bacteria, denitrifiers, and anammox organisms. 
      This study conclusively demonstrates that microbial communities are well-adapted 
      to Bauxsol pellets and bacterial communities involved in the nitrogen cycle are 
      present.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Despland, Laure M
AU  - Despland LM
AD  - School of Environment, Science & Engineering, Southern Cross University, P.O. Box 
      157, Lismore, NSW 2480, Australia. laure.despland@unine.ch
FAU - Vancov, Tony
AU  - Vancov T
FAU - Aragno, Michel
AU  - Aragno M
FAU - Clark, Malcolm W
AU  - Clark MW
LA  - eng
PT  - Journal Article
DEP - 20120720
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Waste Water)
SB  - IM
MH  - Biodiversity
MH  - DNA, Ribosomal/genetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Wastewater
MH  - *Water Microbiology
EDAT- 2012/07/24 06:00
MHDA- 2012/12/27 06:00
CRDT- 2012/07/24 06:00
PHST- 2012/04/19 00:00 [received]
PHST- 2012/06/22 00:00 [revised]
PHST- 2012/06/24 00:00 [accepted]
PHST- 2012/07/24 06:00 [entrez]
PHST- 2012/07/24 06:00 [pubmed]
PHST- 2012/12/27 06:00 [medline]
AID - S0048-9697(12)00912-6 [pii]
AID - 10.1016/j.scitotenv.2012.06.079 [doi]
PST - ppublish
SO  - Sci Total Environ. 2012 Sep 1;433:383-9. doi: 10.1016/j.scitotenv.2012.06.079. 
      Epub 2012 Jul 20.