PMID- 22820188
OWN - NLM
STAT- MEDLINE
DCOM- 20130221
LR  - 20211203
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 425
IP  - 1
DP  - 2012 Aug 17
TI  - TNF-alpha promotes human retinal pigment epithelial (RPE) cell migration by inducing 
      matrix metallopeptidase 9 (MMP-9) expression through activation of Akt/mTORC1 
      signaling.
PG  - 33-8
LID - 10.1016/j.bbrc.2012.07.044 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) promotes in vitro retinal pigment epithelial 
      (RPE) cell migration to initiate proliferative vitreoretinopathy (PVR). Here we 
      report that TNF-alpha promotes human RPE cell migration by inducing matrix 
      metallopeptidase 9 (MMP-9) expression. Inhibition of MMP-9 by its inhibitor or 
      its neutralizing antibody inhibited TNF-alpha-induced in vitro RPE cell migration. 
      Reversely, exogenously-added active MMP-9 promoted RPE cell migration. 
      Suppression Akt/mTOR complex 1(mTORC1) activation by LY 294002 and rapamycin 
      inhibited TNF-alpha-mediated MMP-9 expression. To introduce a constitutively active 
      Akt (CA-Akt) in cultured RPE cells increased MMP-9 expression, and to block 
      mTORC1 activation by rapamycin inhibited its effect. RNA interference 
      (RNAi)-mediated silencing of SIN1, a key component of mTOR complex 2 (mTORC2), 
      had no effect on MMP-9 expression or secretion. In conclusion, this study suggest 
      that TNF-alpha promotes RPE cell migration by inducing MMP-9 expression through 
      activation of Akt/ mTORC1, but not mTORC2 signaling.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Wang, Cheng-hu
AU  - Wang CH
AD  - The Affiliated Eye Hospital of Nanjing Medical University, Nanjing 210029, China.
FAU - Cao, Guo-Fan
AU  - Cao GF
FAU - Jiang, Qin
AU  - Jiang Q
FAU - Yao, Jin
AU  - Yao J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120720
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (MAPKAP1 protein, human)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/genetics/metabolism
MH  - Cell Movement/*drug effects/physiology
MH  - Cells, Cultured
MH  - Humans
MH  - Matrix Metalloproteinase 9/*biosynthesis
MH  - Mechanistic Target of Rapamycin Complex 1
MH  - Multiprotein Complexes
MH  - Pigment Epithelium of Eye/*drug effects/enzymology/physiology
MH  - Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-akt/*biosynthesis
MH  - RNA Interference
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Vitreoretinopathy, Proliferative/enzymology/pathology
EDAT- 2012/07/24 06:00
MHDA- 2013/02/22 06:00
CRDT- 2012/07/24 06:00
PHST- 2012/06/13 00:00 [received]
PHST- 2012/07/11 00:00 [accepted]
PHST- 2012/07/24 06:00 [entrez]
PHST- 2012/07/24 06:00 [pubmed]
PHST- 2013/02/22 06:00 [medline]
AID - S0006-291X(12)01340-X [pii]
AID - 10.1016/j.bbrc.2012.07.044 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 Aug 17;425(1):33-8. doi: 
      10.1016/j.bbrc.2012.07.044. Epub 2012 Jul 20.