PMID- 22820903
OWN - NLM
STAT- MEDLINE
DCOM- 20130322
LR  - 20220409
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 404
IP  - 5
DP  - 2012 Sep
TI  - Preparation of a magnetic molecularly imprinted polymer with pseudo template for 
      rapid simultaneous determination of cyromazine and melamine in bio-matrix 
      samples.
PG  - 1555-64
LID - 10.1007/s00216-012-6200-7 [doi]
AB  - A magnetic molecularly imprinted polymer (M-MIP) for cyromazine and melamine was 
      prepared by simple suspension polymerization using a pseudo template, 
      2-(4,6-diamino-1,3,5-triazin-2-ylamino)ethanethiol disulfide. The M-MIP was 
      characterized by scanning electron microscopy, Fourier-transform infrared 
      spectroscopy, and vibrating sample magnetometry. Molecular recognition properties 
      and binding capability to cyromazine and melamine were evaluated by adsorption 
      testing, which showed the M-MIP had better affinity and selectivity than the 
      magnetic non-imprinted polymer (M-NIP) for cyromazine and melamine. A method 
      based on molecularly imprinted solid-phase extraction assisted by magnetic 
      separation was developed for extraction of cyromazine and melamine from 
      bio-matrix samples. Various conditions, for example desorption conditions, amount 
      of M-MIP, extraction time, and sample pH were optimized. High-performance liquid 
      chromatography with UV detection was used to determine cyromazine and melamine 
      after extraction. The proposed method was successfully applied to determination 
      of cyromazine and melamine in egg and milk samples. Recovery of standard spiked 
      cyromazine and melamine from these samples was between 71.86 and 80.57%, with 
      intraday and interday relative standard deviation ranging from 3.45 to 6.39% and 
      from 3.95 to 7.84%, respectively. The results indicate that the pseudo template 
      M-MIP can be used for preconcentration, purification, and analysis of cyromazine 
      and melamine in bio-matrix samples.
FAU - Wang, Xianhua
AU  - Wang X
AD  - School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 
      China.
FAU - Fang, Qiuxue
AU  - Fang Q
FAU - Liu, Shipeng
AU  - Liu S
FAU - Chen, Lei
AU  - Chen L
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120721
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Insecticides)
RN  - 0 (Polymers)
RN  - 0 (Triazines)
RN  - CA49Y29RA9 (cyromazine)
RN  - N3GP2YSD88 (melamine)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Eggs/*analysis
MH  - Insecticides/*isolation & purification
MH  - Magnets/chemistry
MH  - Milk/*chemistry
MH  - *Molecular Imprinting
MH  - Polymerization
MH  - Polymers/*chemistry
MH  - Sensitivity and Specificity
MH  - Solid Phase Extraction/methods
MH  - Triazines/*isolation & purification
EDAT- 2012/07/24 06:00
MHDA- 2013/03/23 06:00
CRDT- 2012/07/24 06:00
PHST- 2012/02/24 00:00 [received]
PHST- 2012/06/14 00:00 [accepted]
PHST- 2012/06/13 00:00 [revised]
PHST- 2012/07/24 06:00 [entrez]
PHST- 2012/07/24 06:00 [pubmed]
PHST- 2013/03/23 06:00 [medline]
AID - 10.1007/s00216-012-6200-7 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2012 Sep;404(5):1555-64. doi: 10.1007/s00216-012-6200-7. Epub 
      2012 Jul 21.