PMID- 22821237
OWN - NLM
STAT- MEDLINE
DCOM- 20121029
LR  - 20211021
IS  - 1544-0591 (Electronic)
IS  - 0022-0345 (Print)
IS  - 0022-0345 (Linking)
VI  - 91
IP  - 9
DP  - 2012 Sep
TI  - Differential expression of HIF-1alpha in skin and mucosal wounds.
PG  - 871-6
LID - 10.1177/0022034512454435 [doi]
AB  - Despite accelerated epithelial closure, oral mucosal wounds exhibit lower levels 
      of VEGF and a more refined angiogenic response than do skin wounds. The specific 
      differences in angiogenesis suggest that skin and oral mucosal wounds may 
      experience dissimilar levels of hypoxia and HIF-1alpha. Using a model of comparable 
      wounds on murine dorsal skin and tongue, we determined levels of hypoxia and 
      HIF-1alpha. Skin wounds were found to be significantly more hypoxic and had higher 
      levels of HIF-1alpha than mucosal wounds. Furthermore, under stressed conditions, 
      skin wounds, but not mucosal wounds, exhibited a further elevation of HIF-1alpha 
      beyond that of non-stressed levels. To determine if manipulation of oxygen levels 
      might equalize the repair response of each tissue, we exposed mice to hyperbaric 
      oxygen treatment (HBOT) following wounding. HBOT did not significantly change 
      HIF-1alpha or VEGF expression in either skin or mucosal wounds, nor did it alter 
      wound bed vascularity. These studies suggest that skin wounds have higher levels 
      of hypoxia than do mucosal wounds, along with a differential expression of 
      HIF-1alpha. Interestingly, modulation of oxygen by HBOT does not ameliorate this 
      difference. These results suggest that differential responses to hypoxia may 
      underlie the distinctive wound-healing phenotypes seen in skin and oral mucosa.
FAU - Chen, L
AU  - Chen L
AD  - Center for Wound Healing and Tissue Regeneration, College of Dentistry, 
      University of Illinois at Chicago, 801 S. Paulina St., MC 859, Chicago, IL 60612, 
      USA.
FAU - Gajendrareddy, P K
AU  - Gajendrareddy PK
FAU - DiPietro, L A
AU  - DiPietro LA
LA  - eng
GR  - P20 GM078426/GM/NIGMS NIH HHS/United States
GR  - R01 GM050875/GM/NIGMS NIH HHS/United States
GR  - P20-GM078426/GM/NIGMS NIH HHS/United States
GR  - R01-GM50875/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120719
PL  - United States
TA  - J Dent Res
JT  - Journal of dental research
JID - 0354343
RN  - 0 (Hif1a protein, mouse)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - Female
MH  - Gene Expression Regulation
MH  - Hyperbaric Oxygenation
MH  - Hypoxia/metabolism/therapy
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*biosynthesis/genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mouth Mucosa/blood supply/*injuries/metabolism
MH  - Neovascularization, Physiologic
MH  - Skin/blood supply/*injuries/metabolism
MH  - Tongue/blood supply/*injuries/metabolism
MH  - Up-Regulation
MH  - Vascular Endothelial Growth Factor A/biosynthesis
MH  - Wound Healing/*physiology
PMC - PMC3420394
COIS- The author(s) declare no potential conflicts of interest with respect to the 
      authorship and/or publication of this article.
EDAT- 2012/07/24 06:00
MHDA- 2012/10/30 06:00
PMCR- 2013/09/01
CRDT- 2012/07/24 06:00
PHST- 2012/07/24 06:00 [entrez]
PHST- 2012/07/24 06:00 [pubmed]
PHST- 2012/10/30 06:00 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - 0022034512454435 [pii]
AID - 10.1177_0022034512454435 [pii]
AID - 10.1177/0022034512454435 [doi]
PST - ppublish
SO  - J Dent Res. 2012 Sep;91(9):871-6. doi: 10.1177/0022034512454435. Epub 2012 Jul 
      19.