PMID- 22823535
OWN - NLM
STAT- MEDLINE
DCOM- 20130823
LR  - 20181202
IS  - 1600-0714 (Electronic)
IS  - 0904-2512 (Linking)
VI  - 42
IP  - 2
DP  - 2013 Feb
TI  - MicroRNA155 in the growth and invasion of salivary adenoid cystic carcinoma.
PG  - 140-7
LID - 10.1111/j.1600-0714.2012.01189.x [doi]
AB  - BACKGROUND: The carcinogenesis mechanism of adenoid cystic carcinoma (ACC) of the 
      salivary gland is poorly understood. MicroRNA155 (miRNA155) has been involved in 
      the carcinogenesis of many malignant tumors. The present study aims to examine 
      the role of miRNA155 in tumor growth and invasion of ACC. METHODS: MiRNA155 
      expression was determined in ACC specimens along with normal salivary glands by 
      quantitative PCR. Using ACC-2 cells as a model for ACC, cell proliferation was 
      examined by MTT assay after knocking down miRNA155 expression, and cell cycle 
      analysis was performed. Invasive capacity of ACC-2 cells was examined by a 
      Transwell culture assay. The effect of miRNA155 on tumor growth was also examined 
      in vivo using mouse models. The effect of miRNA155 on epidermal growth factor 
      receptor (EGFR)/NF-kappaB was studied by quantitative PCR and electrophoretic 
      mobility shift assay. RESULTS: MiRNA155 was over-expressed in ACC. Proliferation 
      of ACC-2 cells was markedly inhibited by knocking down miRNA155, resulting from a 
      blockade of cell cycle in the G1 phase. Inhibition of miRNA155 significantly 
      suppressed the invasive capacity of ACC-2 cells. In vivo growth of ACC-2 
      cell-derived tumors was significantly slower by inhibition of miRNA155. 
      Inhibition of miRNA155 also resulted in decreased expression of EGFR and RelA 
      (NF-kappaB). CONCLUSION: The results suggest that miRNA155 facilitates cell cycle 
      progression and promotes invasion in ACC and that the EGFR/NF-kappaB pathway might 
      participate in mediating the effects of miRNA155. This study has provided 
      insights into the carcinogenic mechanisms of ACC and identified new targets for 
      intervention of salivary ACC.
CI  - (c) 2012 John Wiley & Sons A/S.
FAU - Liu, Liu
AU  - Liu L
AD  - Department of Oral and Maxillofacial Surgery, School of Stomatology, The 9th 
      People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 
      China. cycstal2004@hotmail.com
FAU - Hu, Yongjie
AU  - Hu Y
FAU - Fu, Jingye
AU  - Fu J
FAU - Yang, Xihu
AU  - Yang X
FAU - Zhang, Zhiyuan
AU  - Zhang Z
LA  - eng
PT  - Journal Article
DEP - 20120723
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (Coloring Agents)
RN  - 0 (MIRN155 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-kappa B)
RN  - 0 (RELA protein, human)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 0 (Transcription Factor RelA)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EUY85H477I (thiazolyl blue)
SB  - IM
MH  - Animals
MH  - Carcinoma, Adenoid Cystic/genetics/*pathology/secondary
MH  - Cell Cycle
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cell Transformation, Neoplastic/pathology
MH  - Coloring Agents
MH  - Disease Models, Animal
MH  - Electrophoretic Mobility Shift Assay
MH  - ErbB Receptors/analysis
MH  - Female
MH  - G1 Phase
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/antagonists & inhibitors/*physiology
MH  - Middle Aged
MH  - NF-kappa B/analysis
MH  - Neoplasm Invasiveness
MH  - Parotid Gland/pathology
MH  - Polymerase Chain Reaction
MH  - Salivary Gland Neoplasms/genetics/*pathology
MH  - Tetrazolium Salts
MH  - Thiazoles
MH  - Transcription Factor RelA/analysis
EDAT- 2012/07/25 06:00
MHDA- 2013/08/24 06:00
CRDT- 2012/07/25 06:00
PHST- 2012/07/25 06:00 [entrez]
PHST- 2012/07/25 06:00 [pubmed]
PHST- 2013/08/24 06:00 [medline]
AID - 10.1111/j.1600-0714.2012.01189.x [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2013 Feb;42(2):140-7. doi: 10.1111/j.1600-0714.2012.01189.x. 
      Epub 2012 Jul 23.