PMID- 22824512
OWN - NLM
STAT- MEDLINE
DCOM- 20121207
LR  - 20120822
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 318
IP  - 18
DP  - 2012 Nov 1
TI  - Establishment and characterization of mouse bone marrow-derived mast cell 
      hybridomas.
PG  - 2385-96
LID - 10.1016/j.yexcr.2012.07.010 [doi]
AB  - Interleukin (IL)-3-dependent mouse bone marrow-derived mast cells (BMMCs) are an 
      important model for studying the function of mucosal-type mast cells. In the 
      present study, BMMCs were successfully immortalized by cell fusion using a 
      hypoxanthine-aminopterin-thymidine medium-sensitive variant of P815 mouse 
      mastocytoma (P815-6TgR) as a partner cell line. The established mouse mast cell 
      hybridomas (MMCHs) expressed alpha, beta, and gamma subunits of high-affinity immunoglobulin 
      E (IgE) receptor (FcepsilonRI) and possessed cytoplasmic granules devoid of or 
      partially filled with electron-dense material. Four independent MMCH clones 
      continuously proliferated without supplemental exogenous IL-3 and showed a 
      degranulation response on stimulation with IgE+antigen. Furthermore, histamine 
      synthesis and release by degranulation were confirmed in MMCH-D5, a MMCH clone 
      that showed the strongest degranulation response. MMCH-D5 exhibited elevated 
      levels of IL-3, IL-4, IL-13, granulocyte-macrophage colony-stimulating factor, 
      tumor necrosis factor (TNF)-alpha, and cyclooxygenase 2, and production of 
      prostaglandin D(2) and leukotriene C(4) in response to IgE-induced stimulation. 
      MMCH clones also expressed Toll-like receptors (TLRs) 1, 2, 4, and 6 and showed 
      elevated levels of TNF-alpha expression in response to stimulation with TLR2 and TLR4 
      ligands. The MMCHs established using this method should be suitable for studies 
      on FcepsilonRI- and TLR-mediated effector functions of mast cells.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Kawahara, Takeshi
AU  - Kawahara T
AD  - Integrated Department of Sciences of Functional Foods, Graduate School of 
      Agriculture, Shinshu University, Nagano, Japan. tkawafb@shinshu-u.ac.jp
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120721
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Antigens, CD)
RN  - 0 (Fc(alpha) receptor)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/metabolism
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Cell Degranulation
MH  - Hybridomas/*cytology/metabolism
MH  - Ligands
MH  - Mast Cells/cytology/*metabolism
MH  - Mice
MH  - Receptors, Fc/genetics/metabolism
MH  - Receptors, IgE/immunology/metabolism
MH  - Toll-Like Receptors/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2012/07/25 06:00
MHDA- 2012/12/12 06:00
CRDT- 2012/07/25 06:00
PHST- 2012/05/15 00:00 [received]
PHST- 2012/07/09 00:00 [revised]
PHST- 2012/07/12 00:00 [accepted]
PHST- 2012/07/25 06:00 [entrez]
PHST- 2012/07/25 06:00 [pubmed]
PHST- 2012/12/12 06:00 [medline]
AID - S0014-4827(12)00330-8 [pii]
AID - 10.1016/j.yexcr.2012.07.010 [doi]
PST - ppublish
SO  - Exp Cell Res. 2012 Nov 1;318(18):2385-96. doi: 10.1016/j.yexcr.2012.07.010. Epub 
      2012 Jul 21.