PMID- 2282457
OWN - NLM
STAT- MEDLINE
DCOM- 19910315
LR  - 20190510
IS  - 0007-1188 (Print)
IS  - 0007-1188 (Linking)
VI  - 101
IP  - 1
DP  - 1990 Sep
TI  - L-arginine and arginine analogues: effects on isolated blood vessels and cultured 
      endothelial cells.
PG  - 145-51
AB  - 1. The present study examined effects of arginine (Arg) and various Arg analogues 
      on the vascular tone of rabbit and rat aortic rings, the release of nitrite from 
      cultured bovine aortic endothelial cells and the metabolism of L-Arg in bovine 
      and porcine endothelial cell homogenates. The respective D-enantiomers or 
      N-alpha-benzoyl-L-arginine ethyl ester did not substitute for L-Arg. 2. In bovine 
      aortic endothelial cells, the release of nitrite was only observed in the 
      presence of L-Arg or L-Arg methyl ester in the cell culture medium. 3. In 
      dialyzed homogenates of porcine and bovine aortic endothelial cells, L-Arg was 
      metabolized independently of NADPH and Ca2+ to yield L-ornithine (L-Orn) and 
      L-citrulline (L-Cit). No concomitant nitrite formation was detected. 4. 
      Pretreatment of rabbit and rat aortic rings with L-canavanine (L-Can) or 
      NG-monomethyl-L-Arg (L-NMMA) inhibited ATP- and acetylcholine-induced relaxations 
      (endothelium-dependent) but not glyceryltrinitrate-induced relaxations 
      (endothelium-independent). 5. In rabbit aortic rings, Arg and monomeric Arg 
      analogues induced endothelium-independent relaxations. L-Arg methyl ester induced 
      an endothelium-independent contraction, and L-NMMA induced a relaxation in the 
      absence of endothelium and a contraction in the presence of endothelium. 
      Polymeric basic amino acids such as poly L-Arg induced endothelium-dependent 
      relaxations (inhibited by L-Can), a subsequent refractoriness to 
      endothelium-dependent vasodilators (not prevented by L-Can) and endothelial cell 
      death. 6. We suggest that extracellular L-Arg is essential for the formation of 
      endothelium-derived nitrogen oxides (EDNO). However, Arg and Arg analogues do not 
      exert endothelium-dependent relaxation. L-Can and L-NMMA inhibit 
      endothelium-dependent relaxation, consistent with an inhibition of EDNO formation 
      from L-Arg, but also exert endothelium-independent effects on vascular tone.
FAU - Schmidt, H H
AU  - Schmidt HH
AD  - Institut fur Pharmakologie, Freie Universitat Berlin, F.R.G.
FAU - Baeblich, S E
AU  - Baeblich SE
FAU - Zernikow, B C
AU  - Zernikow BC
FAU - Klein, M M
AU  - Klein MM
FAU - Bohme, E
AU  - Bohme E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Pharmacol
JT  - British journal of pharmacology
JID - 7502536
RN  - 0 (Nitrites)
RN  - 0 (Nitrogen Oxides)
RN  - 27JT06E6GR (omega-N-Methylarginine)
RN  - 29VT07BGDA (Citrulline)
RN  - 94ZLA3W45F (Arginine)
RN  - 967-88-4 (benzoyl L-arginine methyl ester)
RN  - E524N2IXA3 (Ornithine)
RN  - ZTI6C33Q2Q (Guanethidine)
SB  - IM
MH  - Animals
MH  - Arginine/analogs & derivatives/metabolism/*pharmacology
MH  - Cattle
MH  - Citrulline/metabolism
MH  - Endothelium, Vascular/*cytology/drug effects
MH  - Guanethidine/pharmacology
MH  - In Vitro Techniques
MH  - Male
MH  - Muscle, Smooth, Vascular/*drug effects
MH  - Nitrites/pharmacology
MH  - Nitrogen Oxides/metabolism
MH  - Ornithine/metabolism
MH  - Rabbits
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Stereoisomerism
MH  - Swine
MH  - omega-N-Methylarginine
PMC - PMC1917657
EDAT- 1990/09/01 00:00
MHDA- 1990/09/01 00:01
PMCR- 1991/09/01
CRDT- 1990/09/01 00:00
PHST- 1990/09/01 00:00 [pubmed]
PHST- 1990/09/01 00:01 [medline]
PHST- 1990/09/01 00:00 [entrez]
PHST- 1991/09/01 00:00 [pmc-release]
AID - 10.1111/j.1476-5381.1990.tb12104.x [doi]
PST - ppublish
SO  - Br J Pharmacol. 1990 Sep;101(1):145-51. doi: 10.1111/j.1476-5381.1990.tb12104.x.