PMID- 22826477
OWN - NLM
STAT- MEDLINE
DCOM- 20121221
LR  - 20211021
IS  - 1476-1645 (Electronic)
IS  - 0002-9637 (Print)
IS  - 0002-9637 (Linking)
VI  - 87
IP  - 4
DP  - 2012 Oct
TI  - Cytokine responses to novel antigens in a peri-urban population in Brazil exposed 
      to Leishmania infantum chagasi.
PG  - 663-70
LID - 10.4269/ajtmh.2012.12-0180 [doi]
AB  - Visceral leishmaniasis (VL) is fatal if untreated, and there are no vaccines for 
      this disease. High levels of CD4-derived interferon-gamma (IFN-gamma) in the presence of 
      low levels of interleukin-10 (IL-10) predicts vaccine success. Tumor necrosis 
      factor-alpha (TNF-alpha) is also important in this process. We characterized human immune 
      responses in three groups exposed to Leishmania infantum chagasi in Brazil: 1) 
      drug-cured VL patients (recovered VL); 2) asymptomatic persons with positive 
      Leishmania-specific delayed-type hypersensitivity skin reactions (DTH+); and 3) 
      DTH-negative household contacts. Magnitude of DTH correlated with crude 
      Leishmania antigen-driven IFN-gamma, TNF-alpha, and IL-5, but not IL-10. DTH+ persons 
      showed equivalent levels of IFN-gamma, but higher levels of IL-10, to tryparedoxin 
      peroxidase and Leishmania homolog of receptor for activated C kinase compared 
      with recovered VL patients. The IFN-gamma:IL-10 and TNF-alpha:IL-10 ratios were higher in 
      recovered VL patients than in DTH+ persons. Seven of 11 novel candidates (R71, 
      L37, N52, L302.06, M18, J41, and M22) elicited cytokine responses (36-71% of 
      responders) in recovered VL patients and DTH+ persons. This result confirmed 
      their putative status as cross-species vaccine/immunotherapeutic candidates.
FAU - Stober, Carmel B
AU  - Stober CB
AD  - Department of Medicine, University of Cambridge School of Clinical Medicine, 
      Cambridge, United Kingdom. c.stober@doctors.org.uk
FAU - Jeronimo, Selma M B
AU  - Jeronimo SM
FAU - Pontes, Nubia N
AU  - Pontes NN
FAU - Miller, E Nancy
AU  - Miller EN
FAU - Blackwell, Jenefer M
AU  - Blackwell JM
LA  - eng
GR  - R01AI067874-01/AI/NIAID NIH HHS/United States
GR  - P50 AI074321/AI/NIAID NIH HHS/United States
GR  - P50 AI030639/AI/NIAID NIH HHS/United States
GR  - R01 AI067874/AI/NIAID NIH HHS/United States
GR  - P50AI-30639/AI/NIAID NIH HHS/United States
GR  - R01 AI076233/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120723
PL  - United States
TA  - Am J Trop Med Hyg
JT  - The American journal of tropical medicine and hygiene
JID - 0370507
RN  - 0 (Antigens, Protozoan)
RN  - 0 (Cytokines)
RN  - 0 (Protozoan Proteins)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 1.11.1.- (tryparedoxin peroxidase)
SB  - IM
MH  - Animals
MH  - Antigens, Protozoan/*immunology
MH  - Brazil
MH  - Cytokines/*immunology
MH  - Humans
MH  - Hypersensitivity, Delayed/immunology
MH  - Leishmania infantum/*immunology
MH  - Leishmaniasis, Visceral/*immunology/parasitology/physiopathology
MH  - Peroxidases/immunology
MH  - Protozoan Proteins/immunology
MH  - *Urban Population
PMC - PMC3516316
EDAT- 2012/07/25 06:00
MHDA- 2012/12/22 06:00
PMCR- 2013/10/03
CRDT- 2012/07/25 06:00
PHST- 2012/07/25 06:00 [entrez]
PHST- 2012/07/25 06:00 [pubmed]
PHST- 2012/12/22 06:00 [medline]
PHST- 2013/10/03 00:00 [pmc-release]
AID - ajtmh.2012.12-0180 [pii]
AID - 10.4269/ajtmh.2012.12-0180 [doi]
PST - ppublish
SO  - Am J Trop Med Hyg. 2012 Oct;87(4):663-70. doi: 10.4269/ajtmh.2012.12-0180. Epub 
      2012 Jul 23.