PMID- 22827758
OWN - NLM
STAT- MEDLINE
DCOM- 20121213
LR  - 20151119
IS  - 1440-1827 (Electronic)
IS  - 1320-5463 (Linking)
VI  - 62
IP  - 8
DP  - 2012 Aug
TI  - Comparison of HER2 immunohistochemical results using a monoclonal antibody 
      (SV2-61gamma) and a polyclonal antibody (for Dako HercepTest) in advanced gastric 
      cancer.
PG  - 513-7
LID - 10.1111/j.1440-1827.2012.02843.x [doi]
AB  - We compared a monoclonal antibody (SV2-61gamma) and a polyclonal antibody (Dako 
      HercepTest) in immunohistochemical assessments of human epidermal growth factor 
      receptor 2 (HER2) expression in 73 samples of advanced gastric cancer. Results 
      were scored as 0 to 3+, and equivocal or discordant (SV2-61gamma/Dako HercepTest = 
      0/2+, 0/3+, 1+/3+ or 2+/3+) cases were subjected to fluorescence in situ 
      hybridization (FISH) analysis. The frequencies of HER2 scores of 2+ or 3+ were 
      15.1% (11/73) using SV2-61gamma and 38.4% (28/73) using Dako HercepTest. All of the 
      equivocal or discordant cases with a HER2 score of 3+ using Dako HercepTest 
      exhibited amplification of the HER2 gene regardless of the HER2 score determined 
      with SV2-61gamma. The results of the HER2 tests differed according to the antibodies 
      used for immunohistochemistry that preceded FISH analysis, being 15.1% (11/73) 
      using SV2-61gamma and 23.3% (17/73) using Dako HercepTest. Thus, therapeutic 
      decisions might be markedly influenced by the selection of antibody used in the 
      HER2 test.
CI  - (c) 2012 The Authors. Pathology International (c) 2012 Japanese Society of Pathology 
      and Blackwell Publishing Asia Pty Ltd.
FAU - Tamura, Gen
AU  - Tamura G
AD  - Department of Pathology and Laboratory Medicine, Yamagata Prefectural Central 
      Hospital, Yamagata, Japan. gtamura@ypch.gr.jp
FAU - Osakabe, Mitsumasa
AU  - Osakabe M
FAU - Yanagawa, Naoki
AU  - Yanagawa N
FAU - Ogata, Shin-ya
AU  - Ogata SY
FAU - Nomura, Takashi
AU  - Nomura T
FAU - Fukushima, Norimasa
AU  - Fukushima N
FAU - Ito, Yoshimasa
AU  - Ito Y
FAU - Tabuchi, Minori
AU  - Tabuchi M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adenocarcinoma/genetics/*metabolism/pathology
MH  - Antibodies, Monoclonal
MH  - Antibody Specificity
MH  - Biomarkers, Tumor/*metabolism
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Reagent Kits, Diagnostic
MH  - Receptor, ErbB-2/genetics/*metabolism
MH  - Staining and Labeling
MH  - Stomach Neoplasms/genetics/*metabolism/pathology
EDAT- 2012/07/26 06:00
MHDA- 2012/12/14 06:00
CRDT- 2012/07/26 06:00
PHST- 2012/07/26 06:00 [entrez]
PHST- 2012/07/26 06:00 [pubmed]
PHST- 2012/12/14 06:00 [medline]
AID - 10.1111/j.1440-1827.2012.02843.x [doi]
PST - ppublish
SO  - Pathol Int. 2012 Aug;62(8):513-7. doi: 10.1111/j.1440-1827.2012.02843.x.