PMID- 22830034
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20120823
LR  - 20240324
IS  - 2090-3049 (Electronic)
IS  - 2090-3030 (Print)
IS  - 2090-3049 (Linking)
VI  - 2012
DP  - 2012
TI  - Use of the signature Fatty Acid 16:1omega5 as a tool to determine the distribution of 
      arbuscular mycorrhizal fungi in soil.
PG  - 236807
LID - 10.1155/2012/236807 [doi]
LID - 236807
AB  - Biomass estimation of arbuscular mycorrhiza (AM) fungi, widespread plant root 
      symbionts, commonly employs lipid biomarkers, predominantly the fatty acid 
      16:1omega5. We briefly reviewed the application of this signature fatty acid, 
      followed by a case study comparing biochemical markers with microscopic 
      techniques in an arable soil following a change to AM non-host plants after 27 
      years of continuous host crops, that is, two successive cropping seasons with 
      wheat followed by amaranth. After switching to the non-host amaranth, spore 
      biomass estimated by the neutral lipid fatty acid (NLFA) 16:1omega5 decreased to 
      almost nil, whereas microscopic spore counts decreased by about 50% only. In 
      contrast, AM hyphal biomass assessed by the phospholipid (PLFA) 16:1omega5 was 
      greater under amaranth than wheat. The application of PLFA 16:1omega5 as biomarker 
      was hampered by background level derived from bacteria, and further enhanced by 
      its incorporation from degrading spores used as microbial resource. Meanwhile, 
      biochemical and morphological assessments showed negative correlation for spores 
      and none for hyphal biomass. In conclusion, the NLFA 16:1omega5 appears to be a 
      feasible indicator for AM fungi of the Glomales group in the complex field soils, 
      whereas the use of PLFA 16:1omega5 for hyphae is unsuitable and should be restricted 
      to controlled laboratory studies.
FAU - Ngosong, Christopher
AU  - Ngosong C
AD  - Institute of Biology, Ecology Group, Humboldt-Universitat zu Berlin, 
      Philippstrasse 13, 10115 Berlin, Germany.
FAU - Gabriel, Elke
AU  - Gabriel E
FAU - Ruess, Liliane
AU  - Ruess L
LA  - eng
PT  - Journal Article
DEP - 20120704
PL  - Egypt
TA  - J Lipids
JT  - Journal of lipids
JID - 101553819
PMC - PMC3398647
EDAT- 2012/07/26 06:00
MHDA- 2012/07/26 06:01
PMCR- 2012/07/04
CRDT- 2012/07/26 06:00
PHST- 2012/03/28 00:00 [received]
PHST- 2012/05/14 00:00 [accepted]
PHST- 2012/07/26 06:00 [entrez]
PHST- 2012/07/26 06:00 [pubmed]
PHST- 2012/07/26 06:01 [medline]
PHST- 2012/07/04 00:00 [pmc-release]
AID - 10.1155/2012/236807 [doi]
PST - ppublish
SO  - J Lipids. 2012;2012:236807. doi: 10.1155/2012/236807. Epub 2012 Jul 4.